SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.
Abstract |
Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are frequently targets of antibody-mediated immunity. In this study, the properties of SadA, a purported trimeric autotransporter adhesin of Salmonella enterica serovar Typhimurium, were examined. We demonstrated that SadA is exposed on the Salmonella cell surface in vitro and in vivo during infection of mice. Expression of SadA resulted in cell aggregation, biofilm formation, and increased adhesion to human intestinal Caco-2 epithelial cells. Immunization of mice with folded, full-length, purified SadA elicited an IgG response which provided limited protection against bacterial challenge. When anti-SadA IgG titers were enhanced by administering alum-precipitated protein, a modest additional protection was afforded. Therefore, despite SadA having pleiotropic functions, it is not a dominant, protective antigen for antibody-mediated protection against Salmonella.
|
Authors | Dhaarini Raghunathan, Timothy J Wells, Faye C Morris, Robert K Shaw, Saeeda Bobat, Sarah E Peters, Gavin K Paterson, Karina Tveen Jensen, Denisse L Leyton, Jessica M A Blair, Douglas F Browning, John Pravin, Adriana Flores-Langarica, Jessica R Hitchcock, Claudia T P Moraes, Roxane M F Piazza, Duncan J Maskell, Mark A Webber, Robin C May, Calman A MacLennan, Laura J Piddock, Adam F Cunningham, Ian R Henderson |
Journal | Infection and immunity
(Infect Immun)
Vol. 79
Issue 11
Pg. 4342-52
(Nov 2011)
ISSN: 1098-5522 [Electronic] United States |
PMID | 21859856
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Adhesins, Bacterial
- Alum Compounds
- Immunoglobulin G
- Membrane Proteins
- aluminum sulfate
|
Topics |
- Adhesins, Bacterial
(genetics, metabolism)
- Alum Compounds
- Animals
- Bacterial Adhesion
(genetics, physiology)
- Biofilms
- Caco-2 Cells
- Escherichia coli K12
(metabolism)
- Gene Expression Regulation, Bacterial
(physiology)
- Humans
- Immunoglobulin G
- Membrane Proteins
(genetics, metabolism)
- Mice
- Phylogeny
- Salmonella typhimurium
(genetics, metabolism, pathogenicity)
- Virulence
|
|
Join CureHunter, for free Research Interface BASIC access!
Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease.
Find out why thousands of doctors, pharma researchers and patient activists
around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!
|