Abstract |
Immunostimulating complexes ( ISCOMs) were built after treatment of a purified surface protein from Eimeria falciformis sporozoites with a palmitic acid derivation, leading to a high ratio (33-64%) of P27 incorporation in these cage-like structures. P27 kept its antigenicity after incorporation in ISCOMs, which induced, after iterative intubations by the oral route to groups of mice, a systemic IgG response, a local IgA response, and a local enhanced cellular response as demonstrated by lymphoproliferation of mesenteric lymph node cells upon in vitro stimulation with antigen. This immunization (120 micrograms in six oral doses at 2-day intervals) afforded mice a partial protection (60%) against a subsequent 400 oocyst challenge. The reduction in daily oocyst excretion was corroborated by significantly different weight losses between immunized and control mice on days 9 and 10 postinfection and the subsequent death of these control mice. These observations provide the first application of ISCOMs to parasitic intestinal diseases.
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Authors | M Kazanji, F Laurent, P Péry |
Journal | Vaccine
(Vaccine)
Vol. 12
Issue 9
Pg. 798-804
(Jul 1994)
ISSN: 0264-410X [Print] Netherlands |
PMID | 7975858
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Protozoan
- Antigens, Protozoan
- ISCOMs
- Membrane Proteins
- Palmitates
- Protozoan Proteins
- Protozoan Vaccines
- Vaccines, Attenuated
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Topics |
- Administration, Oral
- Animals
- Antibodies, Protozoan
(biosynthesis)
- Antigens, Protozoan
(genetics, immunology, metabolism)
- Blood Chemical Analysis
- Blotting, Western
- Cell Division
(immunology)
- Cells, Cultured
- Coccidiosis
(prevention & control)
- Eimeria
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- ISCOMs
(administration & dosage, immunology, therapeutic use, ultrastructure)
- Intestinal Mucosa
(metabolism)
- Lymphocyte Activation
(immunology)
- Male
- Membrane Proteins
(immunology, metabolism)
- Mice
- Mice, Inbred BALB C
- Microscopy, Electron
- Palmitates
(metabolism)
- Protozoan Proteins
(immunology, metabolism)
- Protozoan Vaccines
(administration & dosage, immunology, metabolism)
- Vaccination
- Vaccines, Attenuated
(administration & dosage, immunology, metabolism)
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