c-myc Gene rearrangements involving gamma immunoglobulin heavy chain gene switch regions in murine plasmacytomas.

Abstract

In murine plasmacytomas, the c-myc gene has frequently been found to undergo rearrangement by virtue of a T(12;15) chromosome translocation. The immunoglobulin heavy chain gene switch region (S alpha) constitutes the target for most of these recombinations particularly in IgA producing plasmacytomas. We sought to identify non-S alpha myc target sites in several IgG producing tumors. The c-myc target in MPC-11 (a BALB/c IgG2b producing plasmacytoma) has been cloned, localized to the Igh-C locus and identified as the gamma 2a heavy chain gene switch region (S gamma 2a). Furthermore, by Southern blot hybridization, we have determined that the S gamma 2b region is the c-myc target in two NZB IgG2b producing plasmacytomas. The potential relation between Ig class expressed and c-myc translocation target is discussed.

• Authors: L J Harris, E F Remmers, P Brodeur, R Riblet, P D'Eustachio, K B Marcu
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 11 Issue 23 Pg. 8303-15 (Dec 10 1983) ISSN: 0305-1048 [Print] England
• PMID: 6324081 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Immunoglobulin Heavy Chains
• DNA Restriction Enzymes

Topics

• Animals
• Base Sequence
• Cell Line
• Cloning, Molecular
• DNA Restriction Enzymes
• Genes
• Immunoglobulin Heavy Chains (genetics)
• Mice
• Mice, Inbred Strains
• Nucleic Acid Hybridization
• Plasmacytoma (genetics, immunology)
• Species Specificity