Study of benzphetamine-N-demethylase in streptozotocin-diabetic mice and rats: evidence for the induction of catalytically and immunologically specific forms of cytochrome P450.

Abstract

Phenobarbital treatment and streptozotocin-diabetes both increase, in mouse and rat microsomes, a benzphetamine-N-demethylase activity which can be inhibited by a specific antibody raised against purified rat phenobarbital-induced cytochrome P-450. However, similar studies performed on cytochrome P-450 A and B fractions separated by DEAE-cellulose chromatography, clearly proved that streptozotocin-diabetes promotes in mice the synthesis of two new species of cytochrome P-450 and that the streptozotocin diabetes-induced forms are different in mouse and rat. No such modifications were observed in the mixed-function oxidase system of congenitally diabetic mice.

• Authors: E Rouer, E Le Provost, J P Leroux
• Journal: Biochimie (Biochimie) 1983 Nov-Dec Vol. 65 Issue 11-12 Pg. 679-83 ISSN: 0300-9084 [Print] France
• PMID: 6201195 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epitopes
• Cytochrome P-450 Enzyme System
• benzphetamine N-demethylase
• Oxidoreductases, N-Demethylating
• Phenobarbital

Topics

• Animals
• Cytochrome P-450 Enzyme System (biosynthesis, immunology)
• Diabetes Mellitus, Experimental (enzymology)
• Enzyme Induction (drug effects)
• Epitopes (immunology)
• Immunodiffusion
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Obese
• Microsomes, Liver (enzymology)
• Oxidoreductases, N-Demethylating (metabolism)
• Phenobarbital (pharmacology)
• Rats
• Rats, Inbred Strains