Effects of Allyl Isothiocyanate on Oxidative and Inflammatory Stress in Type 2 Diabetic Rats.

Abstract

Oxidative stress and inflammation play a crucial role in the pathogenesis and progression of diabetes. Currently, there is a growing need to exploit plant-derived bioactive compounds to support conventional therapies. The purpose of this study was to explore allyl isothiocyanate (AITC) potency in reducing oxidative and inflammatory stress along with its profitable modulation trace element status in pathological conditions such as diabetes. Two weeks of oral AITC treatments (2.5, 5, and 25 mg/kg body weight per day) were evaluated in Wistar rats with diabetes induced by a high-fat diet and streptozotocin. The study included AITC influence on antioxidant factors (SOD, CAT, GST, Nrf2), stress and inflammatory markers (cortisol, CRP, IL-1β, IL-6, TNFα, NF-κB), lipid peroxidation indices (TBARS, -SH groups), and trace element status (Fe, Zn, and Cu) in the detoxification and lymphoid organs. Independently of dose, AITC increased cortisol levels in rat blood serum and decreased total thiol groups (T-SH) and protein-bound thiol groups (PB-SH) collaterally with raised thiobarbituric acid reactive substances (TBARS) in diabetic rat liver. The inflammation and oxidative effects were enhanced by an AITC dose increase. The highest dose of AITC, 25 mg/kg b.w., strongly affected the inflammation process by increasing IL-6, IL-1β, and TNFα in the blood serum, and it upregulated Nrf2 transcription factor with increased SOD, GPx, and GST activities in the liver. AITC showed an equivocal effect on profitable modulation of disturbances in mineral homeostasis in the liver, kidney, and spleen. Our findings revealed that two-week AITC treatment exacerbated oxidative and inflammation status in diabetic rats.

• Authors: Monika Okulicz, Iwona Hertig, Ewelina Król, Tomasz Szkudelski
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 27 Issue 17 (Aug 29 2022) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 36080332 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Interleukin-6
• Isothiocyanates
• NF-E2-Related Factor 2
• Sulfhydryl Compounds
• Thiobarbituric Acid Reactive Substances
• Trace Elements
• Tumor Necrosis Factor-alpha
• allyl isothiocyanate
• Superoxide Dismutase
• Hydrocortisone

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Diabetes Mellitus, Experimental (drug therapy, pathology)
• Diabetes Mellitus, Type 2 (drug therapy)
• Hydrocortisone
• Inflammation (drug therapy)
• Interleukin-6 (pharmacology)
• Isothiocyanates
• NF-E2-Related Factor 2
• Oxidative Stress
• Rats
• Rats, Wistar
• Sulfhydryl Compounds (pharmacology)
• Superoxide Dismutase (pharmacology)
• Thiobarbituric Acid Reactive Substances
• Trace Elements
• Tumor Necrosis Factor-alpha (pharmacology)