Abstract |
Liver diseases are particularly severe health problems, but the options available for preventing and treating them remain limited. Accumulating evidence has shown that there is altered expression of individual histone deacetylase (HDAC) family members in hepatocellular carcinoma cells. In a previous study, we have identified a set of proteins which interact with histone deacetylase 1 and 3 (HDAC1/3) in hepatocellular carcinoma cell lines HepG2 by proteomic approach. This study was designed to investigate the therapeutic potential and expression of HDAC1/3-interacting genes in a human hepatocellular carcinoma cell line (HepG2). Pharmacological and transcriptional inhibition of HDAC1/3 resulted in the suppression of cancer cell proliferation, change of cell morphology, and downregulation of HDAC1/3 genes in HepG2 cells. The pharmacological inhibition also resulted in inhibition of liver cancer cell migration by wound scratch assay. Taken together, the results from this study show that the upregulation of HDAC1/3 in hepatocellular carcinoma resulted in the overexpression of CNOT1, PFDN2/6, and HMG20B, and that these genes could serve as novel molecular targets in liver cancer.
|
Authors | Nouf Al-Yhya, Muhammad Farooq Khan, Rafa Sharaf Almeer, Mana M Alshehri, Mohammed S Aldughaim, Mohammad Ahmed Wadaan |
Journal | Environmental science and pollution research international
(Environ Sci Pollut Res Int)
Vol. 28
Issue 35
Pg. 49000-49013
(Sep 2021)
ISSN: 1614-7499 [Electronic] Germany |
PMID | 33929667
(Publication Type: Journal Article)
|
Copyright | © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
Chemical References |
- CNOT1 protein, human
- HMG20B protein, human
- High Mobility Group Proteins
- Molecular Chaperones
- Transcription Factors
- prefoldin
- HDAC1 protein, human
- Histone Deacetylase 1
- Histone Deacetylases
- histone deacetylase 3
|
Topics |
- Carcinoma, Hepatocellular
(genetics)
- Cell Line
- Cell Line, Tumor
- Cell Proliferation
- Hep G2 Cells
- High Mobility Group Proteins
- Histone Deacetylase 1
- Histone Deacetylases
- Humans
- Liver Neoplasms
(genetics)
- Molecular Chaperones
- Proteomics
- Transcription Factors
|