MicroRNA-132 attenuated cardiac fibrosis in myocardial infarction-induced heart failure rats.

Abstract

The aim of the present study was to determine the effect of microRNA (miR)-132 on cardiac fibrosis in myocardial infarction (MI)-induced heart failure and angiotensin (Ang) II-treated cardiac fibroblasts (CFs). Experiments were carried out in Sprague-Dawley rat treatment with ligation of left coronary artery to induce heart failure, and in CFs administration of Ang II to induce fibrosis. The level of miR-132 was increased in the heart of rats with MI-induced heart failure and the Ang II-treated CFs. In MI rats, left ventricle (LV) ejection fraction, fractional shortening, the maximum of the first differentiation of LV pressure (LV +dp/dtmax) and decline (LV -dp/dtmax) and LV systolic pressure (LVSP) were reduced, and LV end-systolic diameter (LVESD), LV end-diastolic diameter (LVEDD), LV volumes in systole (LVVS) and LV volumes in diastole (LVVD) were increased, which were reversed by miR-132 agomiR but deteriorated by miR-132 antagomiR. The expression levels of collagen I, collagen III, transforming growth factor-β (TGF-β), and α-smooth muscle actin (α-SMA) were increased in the heart of rat with MI-induced heart failure and CFs administration of Ang II. These increases were inhibited by miR-132 agomiR but enhanced by miR-132 antagomiR treatment. MiR-132 inhibited PTEN expression, and attenuated PI3K/Akt signal pathway in CFs. These results indicated that the up-regulation of miR-132 improved the cardiac dysfunction, attenuated cardiac fibrosis in heart failure via inhibiting PTEN expression, and attenuating PI3K/Akt signal pathway. Up-regulation of miR-132 may be a strategy for the treatment of heart failure and cardiac fibrosis.

• Authors: Guoyu Wang, Ruzhu Wang, Zhongbao Ruan, Ling Liu, Yong Li, Li Zhu
• Journal: Bioscience reports (Biosci Rep) Vol. 40 Issue 9 (09 30 2020) ISSN: 1573-4935 [Electronic] England
• PMID: 32885809 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Author(s).

Chemical References

• Cardiotonic Agents
• MIRN132 microRNA, rat
• MicroRNAs
• Angiotensin II

Topics

• Angiotensin II (administration & dosage)
• Animals
• Cardiotonic Agents (pharmacology, therapeutic use)
• Cells, Cultured
• Disease Models, Animal
• Echocardiography
• Fibroblasts (drug effects, pathology)
• Fibrosis
• Heart (drug effects)
• Heart Failure (diagnosis, drug therapy, etiology, pathology)
• Humans
• Male
• MicroRNAs (agonists, antagonists & inhibitors, metabolism)
• Myocardial Infarction (chemically induced, complications, diagnosis, pathology)
• Myocardium (cytology, pathology)
• Primary Cell Culture
• Rats
• Signal Transduction