Direct Comparison of Therapeutic Effects on Diabetic Polyneuropathy between Transplantation of Dental Pulp Stem Cells and Administration of Dental Pulp Stem Cell-Secreted Factors.

Abstract

Stem cell transplantation is a potential novel therapy for diabetic polyneuropathy. Dental pulp stem cells (DPSCs) are attractive stem cell sources because DPSCs can be isolated from extracted teeth and cryopreserved while retaining viability. In this study, we directly compared the efficacy of the transplantation of DPSCs and the administration of the secreted factors from DPSCs (DPSC-SFs) on diabetic polyneuropathy. Eight weeks after streptozotocin injection, DPSCs (1.0 × 106 cells/rat) or DPSC-SFs (1.0 mL/rat) were administered into the unilateral hindlimb skeletal muscles of diabetic Sprague-Dawley rats. DPSC transplantation and DPSC-SF administration did not affect blood glucose levels and body weights in the diabetic rats. Both DPSC transplantation and DPSC-SF administration significantly ameliorated sciatic nerve conduction velocity and sciatic nerve blood flow, accompanied by increases in muscle bundle size, vascular density in the skeletal muscles and intraepidermal nerve fiber density in the diabetic rats, while there was no difference between the results for DPSCs and DPSC-SFs. These results suggest that the efficacy of both DPSC transplantation and DPSC-SF administration for diabetic polyneuropathy four weeks after transplantation/administration was mainly due to the multiple secretomes secreted from transplanted DPSCs or directly injected DPSC-SFs in the early phase of transplantation/administration.

• Authors: Saki Kanada, Eriko Makino, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Masaki Hata, Taisuke Yamauchi, Noritaka Sawada, Shun Kondo, Tomokazu Saiki, Tomomi Minato, Ken Miyazawa, Shigemi Goto, Tatsuaki Matsubara, Keiko Naruse
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 17 (Aug 23 2020) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 32842469 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Blood Glucose
• Culture Media, Conditioned
• Nerve Growth Factors

Topics

• Animals
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Culture Media, Conditioned (chemistry, metabolism, pharmacology)
• Dental Pulp (cytology)
• Diabetes Mellitus, Experimental (complications)
• Diabetic Neuropathies (etiology, therapy)
• Hindlimb
• Male
• Motor Neurons (drug effects, physiology)
• Muscle, Skeletal (drug effects, physiopathology)
• Nerve Fibers (pathology)
• Nerve Growth Factors (genetics)
• Neural Conduction (drug effects)
• Rats, Sprague-Dawley
• Sciatic Nerve (blood supply, drug effects, physiopathology)
• Stem Cell Transplantation (methods)
• Stem Cells (metabolism)