Abstract | BACKGROUND:
Hyperlipidemia triggers atherosclerosis by involving immune cells, such as T-cells. T-cells plays a role in worsening conditions during a high-fat diet (HFD). OBJECTIVE: The research aimed to analyze the role of single garlic oil (SGO) on T-cells activation and its proinflammatory cytokine expression in HFD mice. METHODS: Mice were divided into six groups: ND (normal diet); HFD (high-fat diet without treatment); HFD + Simv (HFD + simvastatin 2.6 mg/kg body weight); and HFD + SGO 1-3 (high-fat diet + single garlic oil in a dose of 12.5, 25, and 50 mg/kg body weight), respectively. Treatments were orally given every day for 45 days. At the end of treatments, lymphocytes were isolated from mice spleen. The relative number of T-cells and proinflammatory cytokines were measured using flow-cytometry and analyzed using one-way ANOVA (p < 0.05). RESULT: Our result indicated that HFD mice had lower naive T cells (CD4+CD62L+) than normal mice (p < 0.05). SGO treatment in HFD mice increased the relative number of naïve T cells. HFD treatment increased the expression of TNF-α and IFN-γ through NF-κB expression. Furthermore, SGO treatment improved the expression of TNF-α and IFN-γ. CONCLUSION: Our study suggests that SGO could act as a promising prospect for therapy to improve chronic inflammation in a HFD.
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Authors | Sri Rahayu Lestari, Mochammad Fitri Atho'illah, Yuyun Ika Christina, Muhaimin Rifa'i |
Journal | Journal of Ayurveda and integrative medicine
(J Ayurveda Integr Med)
2020 Oct-Dec
Vol. 11
Issue 4
Pg. 414-420
ISSN: 0975-9476 [Print] United States |
PMID | 32798194
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved. |