Probiotic Bifidobacterium breve prevents DOCA-salt hypertension.

Abstract

Many probiotics that affect gut microbial ecology have been shown to produce beneficial effects on renin-angiotensin-dependent rodent models and human hypertension. We hypothesized that Bifidobacterium breve CECT7263 (BFM) would attenuate hypertension in deoxycorticosterone acetate (DOCA)-salt rats, a renin-independent model of hypertension. Rats were randomly divided into five groups: control, DOCA-salt, treated DOCA-salt-BFM, treated DOCA-salt-butyrate, and treated DOCA-salt-acetate, for 5 weeks. BFM prevented the increase in systolic blood pressure, cardiac weight, and renal damage induced by DOCA-salt. BFM increased acetate-producing bacterial population and gut acetate levels, improved colonic integrity, normalized endotoxemia, plasma trimethylamine (TMA) levels, and restored the Th17 and Treg content in mesenteric lymph nodes and aorta. Furthermore, BFM improved nitric oxide-dependent vasorelaxation induced by acetylcholine in aortic rings and reduced NADPH oxidase activity in DOCA-salt animals. These protective effects were mimicked by acetate, but not by butyrate supplementation. These data demonstrate that BFM induces changes in gut microbiota linked with attenuation of endothelial dysfunction and increase in blood pressure in this low-renin form of hypertension. These beneficial effects seem to be mediated by increased acetate and reduced TMA production by gut microbiota, thus, improving gut integrity and restoring Th17/Tregs polarization and endotoxemia.

• Authors: Iñaki Robles-Vera, Néstor de la Visitación, Marta Toral, Manuel Sánchez, Miguel Romero, Manuel Gómez-Guzmán, Tao Yang, José L Izquierdo-García, Eduardo Guerra-Hernández, Jesús Ruiz-Cabello, Mohan K Raizada, Francisco Pérez-Vizcaíno, Rosario Jiménez, Juan Duarte
• Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 34 Issue 10 Pg. 13626-13640 (10 2020) ISSN: 1530-6860 [Electronic] United States
• PMID: 32780919 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Federation of American Societies for Experimental Biology.

Chemical References

• Desoxycorticosterone Acetate

Topics

• Animals
• Bifidobacterium breve
• Blood Pressure
• Desoxycorticosterone Acetate
• Gastrointestinal Microbiome
• Hypertension (chemically induced, therapy)
• Male
• Probiotics (therapeutic use)
• Rats
• Rats, Wistar
• Vasodilation