Sulfated galactofucan from Sargassum thunbergii induces senescence in human lung cancer A549 cells.

Abstract

Isolated compounds from Sargassum thunbergii (S. thunbergii) have shown to exhibit diverse biological activities, including anti-cancer activity. In this study, we examined the effect of sulfated galactofucan (SWZ-4-H), which was successfully isolated from S. thunbergii, and its underlying mechanism on human lung cancer (LC) A549 cell growth in vitro and in vivo. In vitro experiment indicated that SWZ-4-H decreased cell growth and number in a dose-dependent manner (P < 0.05 vs. control). Besides, cells treated with SWZ-4-H had irregular morphology, including increased cell volumes, and large nuclei, which suggested senescence-like changes. Moreover, SWZ-4-H increased senescence-related β-galactosidase (SA-β-Gal) staining in a dose-dependent manner; however, while lower (1 mg mL-1) concentration induced mainly senescence without causing cell death, higher dosage (3 mg mL-1) induced both senescence and cell death. The effect of SWZ-4-H was further confirmed by analyzing the expression of p53, p21, p16, and Rb (p-RB); SWZ-4-H significantly increased the expression of p53, p21, and p16 and decreased phosphorylated Rb (p-RB) in a dose-dependent manner. Moreover, in vivo experiment showed that SWZ-4-H significantly reduced the tumor volume without affecting the body weight. To sum up, our data indicated that SWZ-4-H could induce lung cancer senescence by regulating p53, p21, p16, and p-Rb, thus providing a novel perspective on anti-cancer mechanisms of SWZ-4-H in human lung cancer A549 cells.

• Authors: Yizhong Bao , Xinyue He , Wanli Wu , Sanying Wang , Jihuan Dai , Zhongshan Zhang , Weihua Jin , Jing Yan , Genxiang Mao
• Journal: Food & function (Food Funct) Vol. 11 Issue 5 Pg. 4785-4792 (May 01 2020) ISSN: 2042-650X [Electronic] England
• PMID: 32421130 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Plant Extracts

Topics

• A549 Cells (drug effects)
• Antineoplastic Agents (administration & dosage, pharmacology, therapeutic use)
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Humans
• Lung Neoplasms (drug therapy)
• Phytotherapy
• Plant Extracts (administration & dosage, pharmacology, therapeutic use)
• Sargassum