Construction of a Stapled α-Helix Peptide Library Displayed on Phage for the Screening of Galectin-3-Binding Peptide Ligands.

Abstract

A stapled α-helix peptide library was designed and constructed using a chemically modified phage display system for screening stapled-peptide ligands against target proteins. The α-helix peptide library, with two cysteine residues on the opposite side of the randomized face, was modified with a rigid hydrocarbon staple linker on a phage. The stapled α-helix peptide phage library was screened against galectin-3 (Gal-3), a cancer-related galactose-binding protein. The obtained stapled peptides showed a high binding affinity (K d = 0.45 μM) despite being nonsugar ligands. The stapled modification played important roles in stabilizing the α-helical structure that contributed to the high binding affinity to Gal-3. In addition, the best stapled peptide ligands showed specific binding to Gal-3 among various carbohydrate-binding proteins. Thus, the designed α-helix peptide phage library with a constrained structure by the staple linker will advance the discovery of peptide ligands with improved specificity and affinity.

• Authors: Teerapat Anananuchatkul, Iou Ven Chang, Takayuki Miki, Hiroshi Tsutsumi, Hisakazu Mihara
• Journal: ACS omega (ACS Omega) Vol. 5 Issue 11 Pg. 5666-5674 (Mar 24 2020) ISSN: 2470-1343 [Electronic] United States
• PMID: 32226843 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 American Chemical Society.