Anti-HIV antibodies in the CSF of AIDS patients: a serological and immunoblotting study.

Abstract

CSF and serum samples from 16 AIDS patients were tested for the presence of anti-HIV antibodies either by classical serological methods or by an immunoblot technique based on agarose gel isoelectric focusing and transfer of the specific IgG antibodies onto HIV antigens-loaded nitrocellulose sheets. This method enabled the demonstration of an intrathecal synthesis of anti-HIV oligoclonal IgG antibodies, often superimposed on diffuse polyclonal production, in 14 patients. The two negative cases were devoid of neurological signs or symptoms. However, two patients classified in stage II of the disease (asymptomatic infection) displayed an intrathecal synthesis of anti-HIV antibodies.

• Authors: K S Bukasa, C J Sindic, M Bodeus, G Burtonboy, C Laterre, J Sonnet
• Journal: Journal of neurology, neurosurgery, and psychiatry (J Neurol Neurosurg Psychiatry) Vol. 51 Issue 8 Pg. 1063-8 (Aug 1988) ISSN: 0022-3050 [Print] England
• PMID: 3216207 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HIV Antibodies
• Immunoglobulin G

Topics

• Acquired Immunodeficiency Syndrome (diagnosis, immunology)
• Adult
• Aged
• Dementia (diagnosis, immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• HIV (immunology)
• HIV Antibodies (cerebrospinal fluid)
• Humans
• Immunoblotting
• Immunoglobulin G (cerebrospinal fluid)
• Male
• Middle Aged