Inhibitory effects of orientin in mast cell-mediated allergic inflammation.

Abstract

BACKGROUND:

Mast cells are immune effector cells mediating allergic inflammation by the secretion of inflammatory mediators such as histamine and pro-inflammatory cytokines. Orientin is a naturally occurring bioactive flavonoid that possesses diverse biological properties, including anti-inflammation, anti-oxidative, anti-tumor, and cardio protection. The objective of this study was to rule out the effectiveness of orientin in mast cell-mediated allergic inflammation.

METHODS:

In this study, in vitro effects of orientin were evaluated in RBL-2H3, mouse bone marrow-derived mast cells, rat peritoneal mast cells, and in vivo effects were evaluated by inducing passive cutaneous anaphylaxis (PCA) in Imprinting Control Region (ICR) mice.

RESULTS:

Findings show that orientin suppressed the immunoglobulin E (IgE)-mediated mast cell degranulation by reducing intracellular calcium level in a concentration-dependent manner. Orientin suppressed the secretion of pro-inflammatory cytokines in mast cells. This inhibitory effects of orientin was through inhibition of FcεRI-mediated signaling proteins. In addition, oral administration of orientin suppressed the IgE-mediated PCA reactions in a dose-dependent manner, which was evidenced by reduced Evan's blue pigmentation and ear swelling.

CONCLUSIONS:

Based on these findings, we suggest that orientin might have potential to alleviate allergic reaction and mast cell-mediated allergic disease.

• Authors: Hima Dhakal, Soyoung Lee, Jin Kyeong Choi, Taeg Kyu Kwon, Dongwoo Khang, Sang-Hyun Kim
• Journal: Pharmacological reports : PR (Pharmacol Rep) Vol. 72 Issue 4 Pg. 1002-1010 (Aug 2020) ISSN: 2299-5684 [Electronic] Switzerland
• PMID: 32048267 (Publication Type: Journal Article)

Chemical References

• Anti-Allergic Agents
• Anti-Inflammatory Agents
• Flavonoids
• Glucosides
• Inflammation Mediators
• orientin

Topics

• Animals
• Anti-Allergic Agents (pharmacology, therapeutic use)
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Cells, Cultured
• Dose-Response Relationship, Drug
• Flavonoids (pharmacology, therapeutic use)
• Glucosides (pharmacology, therapeutic use)
• Hypersensitivity (immunology, metabolism, prevention & control)
• Inflammation Mediators (antagonists & inhibitors, immunology, metabolism)
• Male
• Mast Cells (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred ICR
• Rats
• Rats, Sprague-Dawley