Cardiac hypertrophy in mice submitted to a swimming protocol: influence of training volume and intensity on myocardial renin-angiotensin system.

Abstract

Exercise promotes physiological cardiac hypertrophy and activates the renin-angiotensin system (RAS), which plays an important role in cardiac physiology, both through the classical axis [angiotensin II type 1 receptor (AT1R) activated by angiotensin II (ANG II)] and the alternative axis [proto-oncogene Mas receptor (MASR) activated by angiotensin-(1-7)]. However, very intense exercise could have deleterious effects on the cardiovascular system. We aimed to analyze the cardiac hypertrophy phenotype and the classical and alternative RAS axes in the myocardium of mice submitted to swimming exercises of varying volume and intensity for the development of cardiac hypertrophy. Male Balb/c mice were divided into three groups, sedentary, swimming twice a day without overload (T2), and swimming three times a day with a 2% body weight overload (T3), totaling 6 wk of training. Both training groups developed similar cardiac hypertrophy, but only T3 mice improved their oxidative capacity. We observed that T2 had increased levels of MASR, which was followed by the activation of its main downstream protein AKT; meanwhile, AT1R and its main downstream protein ERK remained unchanged. Furthermore, no change was observed regarding the levels of angiotensin peptides, in either group. In addition, we observed no change in the ratio of expression of the myosin heavy chain β-isoform to that of the α-isoform. Fibrosis was not observed in any of the groups. In conclusion, our results suggest that increasing exercise volume and intensity did not induce a pathological hypertrophy phenotype, but instead improved the oxidative capacity, and this process might have the participation of the RAS alternative axis.

• Authors: Douglas Dos Santos Soares, Graziela Hünning Pinto, Amanda Lopes, Daniel Sturza Lucas Caetano, Thaiane Gomes Nascimento, Michael E Andrades, Nadine Clausell, Luis E Paim Rohde, Santiago Alonso Tobar Leitão, Andreia Biolo
• Journal: American journal of physiology. Regulatory, integrative and comparative physiology (Am J Physiol Regul Integr Comp Physiol) Vol. 316 Issue 6 Pg. R776-R782 (06 01 2019) ISSN: 1522-1490 [Electronic] United States
• PMID: 31042418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Fragments
• Receptor, Angiotensin, Type 1
• Angiotensin II
• Angiotensin I
• angiotensin I (1-7)

Topics

• Angiotensin I (metabolism)
• Angiotensin II (metabolism)
• Animals
• Cardiomegaly (metabolism, physiopathology)
• Hypertrophy, Left Ventricular (metabolism, physiopathology)
• Male
• Mice, Inbred BALB C
• Myocardium (metabolism, pathology)
• Peptide Fragments (metabolism)
• Physical Conditioning, Animal
• Receptor, Angiotensin, Type 1 (metabolism)
• Renin-Angiotensin System (physiology)
• Swimming
• Ventricular Remodeling (physiology)