Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis.

Abstract

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.

• Authors: Ling Guo, Xiao Jiang, Hao-Yuan Tian, Shang-Jin Yao, Bo-Ya Li, Rong-Jie Zhang, Shu-Sheng Zhang, Xin Sun
• Journal: Journal of food and drug analysis (J Food Drug Anal) Vol. 27 Issue 2 Pg. 518-525 (04 2019) ISSN: 2224-6614 [Electronic] China (Republic : 1949- )
• PMID: 30987723 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019. Published by Elsevier Taiwan LLC.

Chemical References

• DNA Adducts
• benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide

Topics

• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (chemistry)
• Chromatography, Liquid
• DNA Adducts (blood, chemistry)
• Female
• Fetal Blood (chemistry)
• Humans
• Molecular Conformation
• Pregnancy
• Tandem Mass Spectrometry