Evodiamine prevents dextran sulfate sodium-induced murine experimental colitis via the regulation of NF-κB and NLRP3 inflammasome.

Abstract

Evodiamine (EVO), an extraction from the traditional Chinese medicine Evodia rutaecarpa, has been reported to possess anti-inflammatory, anti-tumor and other pharmacological activities. However, the effectiveness of EVO to relieve dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) has not been evaluated. In this study, the protective effects and mechanisms of EVO on DSS-induced UC mice were investigated. The results indicated that treatment with EVO ameliorated DSS-induced UC mice body weight loss, disease activity index (DAI), colon length shortening, colonic pathological damage, and myeloperoxidase (MPO) activity. The production of TNF-α, IL-1β and IL-6 was also significantly inhibited by EVO. Further mechanistic results showed that EVO restrained the inflammation by regulating NF-κB signal and NLRP3 inflammasome. Furthermore, results also showed that EVO contributed to the tight junction (TJ) architecture integrity by modulating the expression of zonula occludens-1 (ZO-1) and occludin during colitis. Surprisingly, treatment with EVO reduced the concentration of plasmatic lipopolysaccharide (LPS) and re-balanced the levels of Escherichia coli and Lactobacillus. These findings suggested that EVO may have a potential protective effect on DSS-induced colitis and may be useful for the prevention and treatment of UC.

• Authors: Peng Shen, Zecai Zhang, Kunpeng Zhu, Hongyang Cao, Jiuxi Liu, Xiaojie Lu, Yanxin Li, Yue Jing, Xin Yuan, Yunhe Fu, Yongguo Cao, Naisheng Zhang
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 110 Pg. 786-795 (Feb 2019) ISSN: 1950-6007 [Electronic] France
• PMID: 30554117 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• NF-kappa B
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nlrp3 protein, mouse
• Plant Extracts
• Quinazolines
• Dextran Sulfate
• evodiamine

Topics

• Animals
• Colitis (chemically induced, metabolism, prevention & control)
• Dextran Sulfate (toxicity)
• Dose-Response Relationship, Drug
• Male
• Mice
• Mice, Inbred C57BL
• NF-kappa B (antagonists & inhibitors, biosynthesis)
• NLR Family, Pyrin Domain-Containing 3 Protein (antagonists & inhibitors, biosynthesis)
• Plant Extracts (pharmacology, therapeutic use)
• Quinazolines (pharmacology, therapeutic use)