Prognostic Significance of 14-3-3ε, Aldo-keto Reductase Family 1 B10 and Metallothionein-1 in Hepatocellular Carcinoma.

Abstract

BACKGROUND/AIM:

Expression of 14-3-3ε is associated with prognostic outcomes of hepatocellular carcinoma (HCC) patients. Metallothionein-1 (MT-1) proteins and aldo-keto-reductase family 1 B10 (AKR1B10) are considered potential tumor regulators of HCC. The aim of this study, was to examine the prognostic value of 14-3-3ε, MT-1 and AKR1B10 expression in HCC.

MATERIALS AND METHODS:

The expression levels of 14-3-3ε, MT-1 and AKR1B10 in HCC cell lines and paraffin-embedded tissues were examined by western blotting and immunohistochemical analysis.

RESULTS:

14-3-3ε positivity was significantly associated with decreased MT-1 expression in HCC. Patients with decreased MT-1 expression had worse survival rates and a higher risk of metastasis than 14-3-3ε-positive HCC patients with unchanged MT-1 expression. Distinct expression patterns of 14-3-3ε/MT-1/AKR1B10 were significantly associated with the metastatic incidence and survival rates of HCC patients. Patients with negative 14-3-3ε staining in primary tumors had better prognostic outcomes. In contrast, patients with positive 14-3-3ε staining, decreased MT-1 expression and no increase in AKR1B10 expression in primary tumors had the worst overall and disease-free survival rates and the highest metastatic risk.

CONCLUSION:

14-3-3ε, AKR1B10, and MT-1 act as potential prognostic biomarkers of HCC.

• Authors: Chih-Ying Wu, Yee-Jee Jan, Bor-Sheng Ko, Ya-Ju Wu, Yi-Ju Wu, Jun-Yang Liou
• Journal: Anticancer research (Anticancer Res) Vol. 38 Issue 12 Pg. 6855-6863 (Dec 2018) ISSN: 1791-7530 [Electronic] Greece
• PMID: 30504401 (Publication Type: Journal Article)
• Copyright: Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• 14-3-3 Proteins
• Biomarkers, Tumor
• Metallothionein
• AKR1B10 protein, human
• Aldo-Keto Reductases
• Aldehyde Reductase

Topics

• 14-3-3 Proteins (metabolism)
• Adult
• Aged
• Aged, 80 and over
• Aldehyde Reductase (metabolism)
• Aldo-Keto Reductases
• Biomarkers, Tumor (metabolism)
• Blotting, Western
• Carcinoma, Hepatocellular (diagnosis, metabolism, pathology)
• Female
• Hep G2 Cells
• Humans
• Immunohistochemistry
• Liver Neoplasms (diagnosis, metabolism, pathology)
• Male
• Metallothionein (metabolism)
• Middle Aged
• Prognosis
• Tumor Cells, Cultured