Glycoprotein modifications of sarcoma L-1 tumor cells by tunicamycin, swainsonine, bromoconduritol or 1-desoxynojirimycin treatment inhibits their metastatic lung colonization in Balb/c-mice.

Abstract

Synthesis and expression of cell surface carbohydrates appear to be involved in recognition events associated with tumor invasion and metastasis. Thus, the potential of murine sarcoma L-1 cells to form experimental lung metastases after i.v. injection was assessed after inhibiting tumor cell protein glycosylation with tunicamycin, swainsonine, bromoconduritol, or 1-desoxynojirimycin. Incubation of sarcoma L-1 cells with 0.5 microgram (or above) of these substances/ml medium for 20-24 h significantly inhibited lung colonization. Cytotoxic side effects or additional organ manifestations could not be found. Gas liquid chromatographic examinations of carbohydrates from treated L-1 cells indicated that sugar synthesis was evidently inhibited. These results suggest that specific glycan structures on tumor cells are required for expression of the metastatic phenotype.

• Authors: G Pulverer, J Beuth, H L Ko, A Yassin, Y Ohshima, K Roszkowski, G Uhlenbruck
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 114 Issue 2 Pg. 217-20 ( 1988) ISSN: 0171-5216 [Print] Germany
• PMID: 2965156 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Carbohydrates
• Cyclohexenes
• Glycoproteins
• Tunicamycin
• 1-Deoxynojirimycin
• 6-bromoconduritol
• Inositol
• Glucosamine
• Swainsonine

Topics

• 1-Deoxynojirimycin
• Alkaloids (pharmacology)
• Animals
• Carbohydrates (analysis)
• Cyclohexenes
• Glucosamine (analogs & derivatives, pharmacology)
• Glycoproteins (analysis, physiology)
• Inositol (analogs & derivatives, pharmacology)
• Lung Neoplasms (secondary)
• Male
• Mice
• Mice, Inbred BALB C
• Sarcoma, Experimental (pathology)
• Swainsonine
• Tunicamycin (pharmacology)