Abstract | BACKGROUND: Nonviral polymer-based gene transfer represents an adaptable system for tumor-targeted gene therapy because various design strategies of shuttle systems, together with the mechanistic concept of active tumor targeting, lead to improved gene delivery vectors resulting in higher tumor specificity, efficacy and safety. METHODS: RESULTS: In vitro incubation of three different tumor cell lines with LPEI-PEG-B6/NIS resulted in significant increase in iodide uptake activity compared to untargeted and empty vectors. After establishment of subcutaneous HuH7 tumors, NIS-conjugated nanoparticles were injected intravenously followed by analysis of radioiodide biodistribution using 123 I-scintigraphy showing significant perchlorate-sensitive iodide accumulation in tumors of LPEI-PEG-B6/NIS-treated mice (8.0 ± 1.5% ID/g 123 I; biological half-life of 4 h). After four cycles of repetitive polyplex/131 I applications, a significant delay of tumor growth was observed, which was associated with markedly improved survival in the therapy group. CONCLUSIONS: These results clearly demonstrate that systemic in vivo NIS gene transfer using nanoparticle vectors coupled to B6 tumor targeting ligand is capable of inducing tumor-specific radioiodide uptake. This promising gene therapy approach opens the exciting prospect of NIS-mediated radionuclide therapy in metastatic cancer, together with the possibility of combining several targeting ligands to enhance selective therapeutic efficacy in a broad field of cancer types with various receptor expression profiles.
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Authors | Sarah Urnauer, Kathrin Klutz, Geoffrey K Grünwald, Stephan Morys, Nathalie Schwenk, Christian Zach, Franz-Josef Gildehaus, Wolfgang Rödl, Manfred Ogris, Ernst Wagner, Christine Spitzweg |
Journal | The journal of gene medicine
(J Gene Med)
Vol. 19
Issue 5
(May 2017)
ISSN: 1521-2254 [Electronic] England |
PMID | 28423213
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 John Wiley & Sons, Ltd. |
Chemical References |
- B6 peptide
- Iodine Radioisotopes
- Oligopeptides
- Polymers
- Symporters
- Polyethylene Glycols
- sodium-iodide symporter
- Polyethyleneimine
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Topics |
- Animals
- Carcinoma, Hepatocellular
(genetics, therapy)
- Cell Line, Tumor
- Female
- Gene Transfer Techniques
- Genetic Therapy
(methods)
- Humans
- Iodine Radioisotopes
(chemistry)
- Liver Neoplasms
(genetics, therapy)
- Mice
- Mice, Nude
- Oligopeptides
(genetics)
- Polyethylene Glycols
(chemistry)
- Polyethyleneimine
(chemistry)
- Polymers
(chemistry)
- Symporters
(chemistry, genetics)
- Tissue Distribution
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