HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Low-dose sustained-release deoxycorticosterone acetate-induced hypertension in Bama miniature pigs for renal sympathetic nerve denervation.

Abstract
Recently, the effectiveness of renal sympathetic nerve denervation for treatment of hypertension has been doubted after SYMPLICITY HTN-3 trial. An ideal animal model is still unavailable for preclinical study about catheter-based renal sympathetic nerve denervation for treatment of hypertension. Traditional high-dose deoxycorticosterone acetate (DOCA)-induced hypertension pig model has some problems due to extensive end-organ damage. Based on the similarity in the anatomic characteristics of renal artery between pigs and humans, this study was undertaken to establish a low-dose sustained-release DOCA-induced hypertension model in pigs. A total of 14 pigs were subcutaneously implanted with low-dose DOCA in the abdomen and cannulated from the femoral artery for the measurement of blood pressure (BP). Plasma angiotensin I (Ang I), angiotensin II (Ang II), plasma renin activity (PRA), aldosterone (Ald), creatinine, epinephrine, and norepinephrine (NE) were determined before and after treatments. The kidneys were collected and processed for hematoxylin and eosin staining, Masson-Goldner trichromic, and periodic acid Schiff staining. Ten pigs survived for 1 month. Mean BP significantly increased after 2-week treatment (P < .001). The plasma Ang I, Ang II, PRA, and Ald significantly decreased (Ang I: 6.92 ± 6.06 vs. 2.22 ± 3.08, P = .002; Ang II: 768.85 ± 525.8 vs. 213.76 ± 148.63, P = .003; PRA: 1.68 ± 1.67 vs. 0.29 ± 0.39, P = .008; Ald: 0.37 ± 0.12 vs. 0.25 ± 0.09, P < .001), but norepinephrine significantly increased (7.59 ± 4.57 vs. 16.96 ± 10.38, P = .021). Plasma creatinine remained unchanged. Hisotological examination showed mild damage to the kidney. Low-dose sustained-release DOCA is able to induce hypertension in pigs. A femoral catheter is not only helpful for monitoring BP, but can be used to quickly exchange the renal sympathetic nerve denervation equipment.
AuthorsWenzheng Han, Weiyi Fang, Qian Gan, Shaofeng Guan, Yanjie Li, Ming Wang, Kaizheng Gong, Xinkai Qu
JournalJournal of the American Society of Hypertension : JASH (J Am Soc Hypertens) Vol. 11 Issue 5 Pg. 314-320 (May 2017) ISSN: 1878-7436 [Electronic] United States
PMID28411074 (Publication Type: Journal Article)
CopyrightCopyright © 2017 American Society of Hypertension. Published by Elsevier Inc. All rights reserved.
Chemical References
  • Delayed-Action Preparations
  • Drug Implants
  • Mineralocorticoids
  • Desoxycorticosterone
Topics
  • Animals
  • Blood Pressure (drug effects)
  • Blood Pressure Determination (methods)
  • Catheters
  • Delayed-Action Preparations (pharmacology)
  • Desoxycorticosterone (pharmacology)
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Implants (pharmacology)
  • Femoral Artery (surgery)
  • Humans
  • Hypertension (blood, chemically induced, surgery)
  • Kidney (innervation, pathology)
  • Kidney Function Tests
  • Male
  • Mineralocorticoids (pharmacology)
  • Renin-Angiotensin System (drug effects)
  • Swine
  • Swine, Miniature (physiology)
  • Sympathectomy

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: