Abstract | BACKGROUND: OBJECTIVE: The aim of this study was to investigate the bio-transformation of selenium by Lactobacillus bacteria towards the characterisation of selenium metabolites. MATERIAL AND METHODS: The speciation of selenium was evaluated by high performance liquid chromatography with inductively coupled plasma mass spectrometry detector. The extraction of selenium species from lyophilized bacteria was executed with water, the mixture of lipase and protease, as well as lisozyme and sodium dodecyl sulphate. RESULTS: All investigated bacteria strains cultivated in the presence of Na2SeO3 effectively uptake selenium. Surprisingly, none of the applied extraction media exhibited a strong power to release the majority of the uptaken selenium compounds. Thus a maximum of 10% of the selenium was extracted from bacteria exposed to the enzymes. However, it was found that Lactobacillus bacteria are able to metabolize inorganic ions of selenium (IV) into Se- methionine, Se-methyloselenocysteine and other unidentified forms. CONCLUSIONS: The study confirmed the ability of probiotic bacteria to biotransform inorganic selenium into its organic derivatives. Therefore, Se-enriched bacteria can be considered as an addition to the functional food. KEY WORDS:
selenium speciation, extraction procedure, Lactobacillus casei bacteria, Lactic acid bacteria (LAB), HPLC ICP-MS, functional food.
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Authors | Eliza Kurek, Anna Ruszczyńska, Marcin Wojciechowski, Anna Łuciuk, Magdalena Michalska-Kacymirow, Ilona Motyl, Ewa Bulska |
Journal | Roczniki Panstwowego Zakladu Higieny
(Rocz Panstw Zakl Hig)
2016
Vol. 67
Issue 3
Pg. 253-62
ISSN: 0035-7715 [Print] Poland |
PMID | 27546322
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Chromatography, High Pressure Liquid
- Cultured Milk Products
(microbiology)
- Dairy Products
(microbiology)
- Humans
- Lactobacillus
(metabolism)
- Saccharomyces cerevisiae
(metabolism)
- Selenium
(metabolism)
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