Protective effects of a Chotosan Fraction and its active components on β-amyloid-induced neurotoxicity.

Abstract

Chotosan (CTS) is a traditional Kampo prescription used to treat chronic headache and hypertension. Recent clinical studies demonstrated that CTS has ameliorative effects on dementia. This study aims to identify the anti-Alzheimer components in CTS. β-amyloid (Aβ) is considered to play a central role in the pathophysiology of Alzheimer's disease. CTS-E, a fraction of CTS, showed significant protective effects on Aβ-induced neurotoxicity. High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used for the qualitative analysis of it. Among the identified constituents, neuroprotective effects against Aβ(25-35)-induced neurotoxicity of 10 major compounds were tested by MTT assay. Their inhibitory action on Aβ(1-42) self-induced aggregation was measured by Thioflavin T-binding assay. The results showed that caffeic acid, chlorogenic acid, 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid had significant neuroprotective effects on Aβ(25-35)-induced neurotoxicity. Besides these phenolic acids, nobiletin and hesperidin could also inhibit Aβ(1-42) self-induced aggregation. In conclusion, the neuroprotective fraction, CTS-E, could protect PC12 cells from Aβ-induced neurotoxicity. Anti-oxidative effects may at least partly mediate the neuroprotective effects of it. Phenolic acids from Chrysanthemi Flos and flavonoids from Citri Reticulatae Pericarpium might be the effective constituents in CTS-E.

• Authors: Menglin Wei, Lei Chen, Jiazhuo Liu, Jiaojiao Zhao, Wenyuan Liu, Feng Feng
• Journal: Neuroscience letters (Neurosci Lett) Vol. 617 Pg. 143-9 (Mar 23 2016) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 26876445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Drugs, Chinese Herbal
• Peptide Fragments
• Protein Aggregates
• Reactive Oxygen Species
• amyloid beta-protein (1-42)
• amyloid beta-protein (25-35)
• choto-san

Topics

• Amyloid beta-Peptides (metabolism, toxicity)
• Animals
• Cell Death
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Neurons (cytology, drug effects)
• PC12 Cells
• Peptide Fragments (metabolism, toxicity)
• Protein Aggregates
• Rats
• Reactive Oxygen Species (metabolism)