Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry.

Abstract

A new mass spectrometry imaging approach to simultaneously map the two-dimensional distribution of N-glycans in tissues has been recently developed. The method uses Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS) to spatially profile the location and distribution of multiple N-linked glycan species released by peptide N-glycosidase F in frozen or formalin-fixed tissues. Multiple formalin-fixed human hepatocellular carcinoma tissues were evaluated with this method, resulting in a panel of over 30 N-glycans detected. An ethylation reaction of extracted N-glycans released from adjacent slides was done to stabilize sialic acid containing glycans, and these structures were compared to N-glycans detected directly from tissue profiling. In addition, the distribution of singly fucosylated N-glycans detected in tumor tissue microarray cores were compared to the histochemistry staining pattern of a core fucose binding lectin. As this MALDI-IMS workflow has the potential to be applied to any formalin-fixed tissue block or tissue microarray, the advantages and limitations of the technique in context with other glycomic methods are also summarized.

• Authors: Thomas W Powers, Stephanie Holst, Manfred Wuhrer, Anand S Mehta, Richard R Drake
• Journal: Biomolecules (Biomolecules) Vol. 5 Issue 4 Pg. 2554-72 (Oct 15 2015) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 26501333 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Polysaccharides
• Formaldehyde

Topics

• Carcinoma, Hepatocellular (metabolism)
• Formaldehyde
• Glycosylation
• Humans
• Liver Neoplasms (metabolism)
• Polysaccharides (analysis, metabolism)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)