DNA Aptamer Raised against Advanced Glycation End Products Prevents Abnormalities in Electroretinograms of Experimental Diabetic Retinopathy.

Abstract

PURPOSE:

Abnormalities in electroretinograms (ERG), such as reduced amplitudes and delayed implicit times of a- and b-wave and oscillatory potentials (OPs), are one of the earliest features of diabetic retinopathy prior to obvious vascular changes in diabetic retinas. We have previously shown that serum levels of advanced glycation end products (AGEs) are correlated with a delayed latency of OPs in type 2 diabetic rats. However, the pathological role of AGEs in ERG abnormalities remains unclear. We examined here whether high-affinity DNA aptamer directed against AGEs (AGE-aptamer) prevents ERG abnormalities in experimental type 1 diabetic retinopathy.

METHODS:

Streptozotocin-induced diabetic rats or control rats received continuous intraperitoneal infusion of either AGE-aptamer or control aptamer via an osmotic mini pump for 16 weeks. Anthropometric, metabolic, and hemodynamic variables were measured, and an ERG was performed.

RESULTS:

Although AGE-aptamer did not affect body weight, fasting and random blood glucose, HbA1c, blood pressure, or lipid parameters, it completely prevented the increase in serum AGE levels as well as the reduction of a- and b-wave and OP amplitudes in diabetic rats.

CONCLUSION:

The present study demonstrated for the first time that AGE-aptamer prevents abnormalities in ERG in experimental diabetic retinopathy probably by blocking the harmful effects of AGEs.

• Authors: Satoshi Maeda, Takanori Matsui, Ayako Ojima, Mika Suematsu, Kuniyoshi Kaseda, Yuichiro Higashimoto, Ryoji Yamakawa, Sho-Ichi Yamagishi
• Journal: Ophthalmic research (Ophthalmic Res) Vol. 54 Issue 4 Pg. 175-80 ( 2015) ISSN: 1423-0259 [Electronic] Switzerland
• PMID: 26492350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 S. Karger AG, Basel.

Chemical References

• Aptamers, Nucleotide
• Blood Glucose
• Glycated Hemoglobin A
• Glycation End Products, Advanced

Topics

• Animals
• Aptamers, Nucleotide (administration & dosage)
• Blood Glucose (metabolism)
• Blood Pressure
• Body Weight
• Diabetes Mellitus, Experimental (prevention & control)
• Diabetes Mellitus, Type 1 (prevention & control)
• Diabetic Retinopathy (prevention & control)
• Electroretinography (drug effects)
• Enzyme-Linked Immunosorbent Assay
• Glycated Hemoglobin (metabolism)
• Glycation End Products, Advanced (blood, genetics)
• Infusion Pumps, Implantable
• Lipid Metabolism
• Male
• Polymerase Chain Reaction
• Rats
• Rats, Wistar