Metastasis is the most lethal step of
cancer progression in patients with invasive
melanoma. In most human
cancers, including
melanoma,
tumor dissemination through the lymphatic vasculature provides a major route for
tumor metastasis. Unfortunately, molecular mechanisms that facilitate interactions between
melanoma cells and lymphatic vessels are unknown. Here, we developed an unbiased approach based on molecular mimicry to identify specific receptors that mediate lymphatic endothelial-
melanoma cell interactions and
metastasis. By screening combinatorial
peptide libraries directly on afferent lymphatic vessels resected from
melanoma patients during sentinel lymphatic mapping and lymph node biopsies, we identified a significant cohort of
melanoma and lymphatic surface binding
peptide sequences. The screening approach was designed so that lymphatic endothelium binding
peptides mimic
cell surface proteins on
tumor cells. Therefore, relevant
metastasis and lymphatic markers were biochemically identified, and a comprehensive molecular profile of the lymphatic endothelium during
melanoma metastasis was generated. Our results identified expression of the
phosphatase 2 regulatory subunit A, α-
isoform (PPP2R1A) on the cell surfaces of both
melanoma cells and lymphatic endothelial cells. Validation experiments showed that PPP2R1A is expressed on the cell surfaces of both
melanoma and lymphatic endothelial cells in vitro as well as independent
melanoma patient samples. More importantly, PPP2R1A-PPP2R1A homodimers occur at the cellular level to mediate cell-cell interactions at the lymphatic-
tumor interface. Our results revealed that PPP2R1A is a new
biomarker for
melanoma metastasis and show, for the first time to our knowledge, an active interaction between the lymphatic vasculature and
melanoma cells during
tumor progression.