Biological properties of IDN5174, a new synthetic camptothecin with the open lactone ring.

Abstract

A series of water-soluble camptothecins obtained by linking a spermidine moiety to the 21-position of the open form through an amidic bond have been tested for their biochemical and biological activities. Growth inhibition assay on the human non-small cell lung cancer carcinoma NCI-H460 cell line revealed that the camptothecin analogues were less potent than topotecan and SN38 after 1 hour of treatment. The potency increased after 72 hours of exposure, being similar to that of reference camptothecins. The analysis of topoisomerase I-mediated DNA cleavage using the purified enzyme indicated that the novel camptothecin analogues retained ability to poison topoisomerase I and displayed the same cleavage pattern of SN38. Persistence of the DNA cleavage was comparable with that of SN38. Stabilization of the cleavable complex was not the result of hydrolysis of the N-C bond between polyamine and the drug because no free camptothecin was recovered at the end of DNA cleavage in presence of IDN5174, the analogue selected for detailed studies. IDN5174 exhibited an antitumor activity comparable with that of topotecan and irinotecan against NCI-H460 tumor xenograft. The pharmacokinetics in mice showed a favorable disposition in tumor tissue with low amount of camptothecin detectable in plasma and tumor (around 5-10%), thus supporting the efficacy of intact IDN5174. In conclusion, we found that IDN5174 maintained the biological and antitumor properties, in spite of lack of the closed E ring. The available results support the interpretation that the polyamine linked at the 21-position may allow a favorable drug interaction in the ternary complex.

• Authors: Giovanni L Beretta, Giovanna Petrangolini, Michelandrea De Cesare, Graziella Pratesi, Paola Perego, Stella Tinelli, Monica Tortoreto, Massimo Zucchetti, Roberta Frapolli, Ezia Bello, Carla Manzotti, Gabriele Fontana, Ezio Bombardelli, Arturo Battaglia, Cristian Samorì, Franco Zunino
• Journal: Cancer research (Cancer Res) Vol. 66 Issue 22 Pg. 10976-82 (Nov 15 2006) ISSN: 0008-5472 [Print] United States
• PMID: 17108136 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• IDN5174
• Lactones
• DNA Topoisomerases, Type I
• Spermidine
• Camptothecin

Topics

• Animals
• Antineoplastic Agents, Phytogenic (pharmacokinetics, pharmacology)
• Camptothecin (analogs & derivatives, pharmacokinetics, pharmacology)
• Cell Growth Processes (drug effects)
• Cell Line, Tumor
• DNA Topoisomerases, Type I (metabolism)
• Humans
• Lactones (pharmacokinetics, pharmacology)
• Mice
• Spermidine (analogs & derivatives)
• Xenograft Model Antitumor Assays