Abstract | BACKGROUND: METHODS: RESULTS: The mRNA levels of CLN1 and cathepsins B, D, and L were significantly higher in metastatic lesions than in primary tumors. In the primary tumors, mRNA expressions of CLN2 and cathepsin D were associated with advanced clinical stages (P < .015 and P < .031, respectively). Among the lysosomal enzymes examined, only the mRNA expression of CLN2 in both the primary tumors of all patients and the pT3 tumors was correlated with the presence of liver metastases (P < .0049 and P < .029, respectively). The polyclonal antibody prepared in the current study demonstrated CLN2 overexpression by immunoblotting and immunohistochemistry. CONCLUSIONS: The results indicate that there is a close correlation between CLN2 and CLN1 expression and colorectal carcinoma progression and metastasis and suggest that they may be potential molecular targets.
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Authors | Toshihiko Tsukamoto, Juri Iida, Yoh Dobashi, Taiji Furukawa, Fumio Konishi |
Journal | Cancer
(Cancer)
Vol. 106
Issue 7
Pg. 1489-97
(Apr 01 2006)
ISSN: 0008-543X [Print] United States |
PMID | 16518810
(Publication Type: Journal Article)
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Copyright | Copyright 2006 American Cancer Society. |
Chemical References |
- Membrane Proteins
- RNA, Messenger
- Tripeptidyl-Peptidase 1
- Thiolester Hydrolases
- PPT1 protein, human
- Cathepsins
- Endopeptidases
- Serine Proteases
- Aminopeptidases
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
- TPP1 protein, human
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Aminopeptidases
- Cathepsins
- Cloning, Molecular
- Colorectal Neoplasms
(enzymology, genetics)
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
- Disease Progression
- Endopeptidases
(biosynthesis, genetics, physiology)
- Female
- Gene Expression Profiling
- Humans
- Male
- Membrane Proteins
(biosynthesis, genetics, physiology)
- Middle Aged
- Neoplasm Metastasis
(physiopathology)
- RNA, Messenger
(analysis, biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
- Serine Proteases
- Thiolester Hydrolases
- Tripeptidyl-Peptidase 1
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