Abstract | BACKGROUND: OBJECTIVE: To examine the toxicity of and dose-dependent response for the inhibition of tumor growth for 1alpha-hydroxyvitamin D(2) (1alpha-OH-D(2)), an analogue with reduced systemic toxicity, in the athymic Y-79 mouse model. METHODS: Mice were randomized into treatment and control groups for 5-week toxicity and dose-response studies. Treatment was via oral gavage 5 times per week. Dose-response studies measured tumor inhibition and drug serum levels. Tumor size and body weight were measured weekly together with various criteria for toxicity. Animals were euthanized at the end of the treatment period. Tumors and kidneys were harvested, and serum was analyzed for calcium and drug levels. RESULTS: Doses of 0.1 to 1.2 microg/d were selected on the basis of toxicity studies for the dose-response trial. Tumor weight and volume in the 0.2-microg and 0.3-microg doses were significantly lower than in controls. Mortality rates and kidney calcification in mice treated with doses of 0.1 to 0.3 microg were lower than those observed in studies of calcitriol and vitamin D(2). CONCLUSION:
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Authors | Richard J Grostern, Paul J Bryar, Michele L Zimbric, Soesiawati R Darjatmoko, Boaz J Lissauer, Mary J Lindstrom, Janice M Lokken, Stephen A Strugnell, Daniel M Albert |
Journal | Archives of ophthalmology (Chicago, Ill. : 1960)
(Arch Ophthalmol)
Vol. 120
Issue 5
Pg. 607-12
(May 2002)
ISSN: 0003-9950 [Print] United States |
PMID | 12003610
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Ergocalciferols
- 1 alpha-hydroxyergocalciferol
- 1,25-dihydroxyergocalciferol
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Topics |
- Animals
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Ergocalciferols
(administration & dosage, blood, toxicity)
- Humans
- Kidney
(drug effects)
- Mice
- Mice, Nude
- Random Allocation
- Retinal Neoplasms
(drug therapy, pathology)
- Retinoblastoma
(drug therapy, pathology)
- Transplantation, Heterologous
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