Recombinant fusion protein and DNA vaccines against foot and mouth disease virus infection in guinea pig and swine.

Abstract

In this study, we provide evidence that a recombinant fusion protein containing beta-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.

• Authors: H Huang, Z Yang, Q Xu, Z Sheng, Y Xie, W Yan, Y You, L Sun, Z Zheng
• Journal: Viral immunology (Viral Immunol) Vol. 12 Issue 1 Pg. 1-8 ( 1999) ISSN: 0882-8245 [Print] United States
• PMID: 10333237 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• Peptides
• Recombinant Fusion Proteins
• Vaccines, DNA
• Viral Proteins
• Viral Vaccines
• beta-Galactosidase

Topics

• Animals
• Antibodies, Viral (biosynthesis)
• Aphthovirus (genetics, immunology)
• Foot-and-Mouth Disease (prevention & control)
• Guinea Pigs
• Immunity, Cellular (genetics)
• Peptides (genetics, immunology)
• Plasmids (immunology)
• Recombinant Fusion Proteins (genetics, immunology)
• Swine
• Vaccines, DNA (genetics, immunology)
• Viral Proteins (genetics, immunology)
• Viral Vaccines (genetics, immunology)
• beta-Galactosidase (genetics, immunology)