Abstract |
Aberrant expression of cell surface carbohydrates such as sialyl Lewis X is associated with tumor formation and metastasis. In order to determine the roles of sialyl Lewis X in tumor metastasis, mouse melanoma B16-F1 cells were stably transfected with alpha1, 3-fucosyltransferase III to express sialyl Lewis X structures. The transfected B16-F1 cells, B16-FTIII, were separated by cell sorting into three different groups based on the expression levels of sialyl Lewis X. When these transfected cells were injected into tail veins of C57BL/6 mice, B16-FTIII.M cells expressing moderate amounts of sialyl Lewis X in poly-N-acetyllactosamines produced large numbers of lung tumor nodules. Surprisingly, B16-FTIII.H cells expressing the highest amount of sialyl Lewis X in shorter N- glycans died in lung blood vessels, producing as few lung nodules as B16-FTIII.N cells which lack sialyl Lewis X. In contrast, B16-FIII.H cells formed more tumors in beige mice and NK cell-depleted C57BL/6 mice than did B16-FTIII.M cells. B16-FTIII.H cells bound to E-selectin better than did B16-FTIII.M cells, but both cells grew at the same rate. These results indicate that excessive expression of sialyl Lewis X in tumor cells leads to rejection by NK cells rather than tumor formation facilitated by attachment to endothelial cells.
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Authors | C Ohyama, S Tsuboi, M Fukuda |
Journal | The EMBO journal
(EMBO J)
Vol. 18
Issue 6
Pg. 1516-25
(Mar 15 1999)
ISSN: 0261-4189 [Print] England |
PMID | 10075923
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- E-Selectin
- Lewis X Antigen
- Oligosaccharides
- Recombinant Proteins
- Sialyl Lewis X Antigen
- Fucosyltransferases
- galactoside 3-fucosyltransferase
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Topics |
- Animals
- Cell Division
- Cytotoxicity, Immunologic
- E-Selectin
(physiology)
- Flow Cytometry
- Fucosyltransferases
(genetics, metabolism)
- Graft Rejection
(immunology, pathology)
- Killer Cells, Natural
(immunology)
- Lewis X Antigen
(immunology)
- Lung Neoplasms
(immunology, pathology, secondary)
- Melanoma, Experimental
(immunology, pathology)
- Mice
- Mice, Inbred C57BL
- Neoplasm Metastasis
- Neoplasm Transplantation
(immunology, pathology)
- Oligosaccharides
(chemistry, immunology)
- Recombinant Proteins
(metabolism)
- Sialyl Lewis X Antigen
- Transfection
- Tumor Cells, Cultured
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