Abstract |
A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)-1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.
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Authors | H S Friedman, S P Johnson, Q Dong, S C Schold, B K Rasheed, S H Bigner, F Ali-Osman, E Dolan, O M Colvin, P Houghton, G Germain, J T Drummond, S Keir, S Marcelli, D D Bigner, P Modrich |
Journal | Cancer research
(Cancer Res)
Vol. 57
Issue 14
Pg. 2933-6
(Jul 15 1997)
ISSN: 0008-5472 [Print] United States |
PMID | 9230204
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Melanocyte-Stimulating Hormones
- Methyltransferases
- O(6)-Methylguanine-DNA Methyltransferase
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Topics |
- Animals
- DNA Methylation
- DNA Repair
- Drug Resistance
- Glioblastoma
(drug therapy, genetics)
- Humans
- Melanocyte-Stimulating Hormones
(analysis)
- Methyltransferases
(metabolism)
- Mice
- Mice, Inbred BALB C
- Microsatellite Repeats
- Neoplasm Transplantation
- O(6)-Methylguanine-DNA Methyltransferase
- Transplantation, Heterologous
- Tumor Cells, Cultured
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