Siglec-F+ neutrophils in the spleen induce immunosuppression following acute infection.

Abstract

Background: The mechanisms underlying the increased mortality of secondary infections during the immunosuppressive phase of sepsis remain elusive. Objectives: We sought to investigate the role of Siglec-F+ neutrophils on splenic T lymphocytes in the immunosuppressed phase of sepsis and on secondary infection in PICS mice, and to elucidate the underlying mechanisms. Methods: We established a mouse model of sepsis-induced immunosuppression followed by secondary infection with LPS or E. coli. The main manifestation of immunosuppression is the functional exhaustion of splenic T lymphocytes. Treg depletion reagent Anti-IL-2, IL-10 blocker Anti-IL-10R, macrophage depletion reagent Liposomes, neutrophil depletion reagent Anti-Ly6G, neutrophil migration inhibitor SB225002, Siglec-F depletion reagent Anti-Siglec-F are all used on PICS mice. The function of neutrophil subsets was investigated by adoptive transplantation and the experiments in vitro. Results: Compared to other organs, we observed a significant reduction in pro-inflammatory cytokines in the spleen, accompanied by a marked increase in IL-10 production, primarily by infiltrating neutrophils. These infiltrating neutrophils in the spleen during the immunosuppressive phase of sepsis undergo phenotypic change in the local microenvironment, exhibiting high expression of neutrophil biomarkers such as Siglec-F, Ly6G, and Siglec-E. Depletion of neutrophils or specifically targeting Siglec-F leads to enhance the function of T lymphocytes and a notable improvement in the survival of mice with secondary infections. Conclusions: We identified Siglec-F+ neutrophils as the primary producers of IL-10, which significantly contributed to T lymphocyte suppression represents a novel finding with potential therapeutic implications.

• Authors: Chaoxiong Liao, Shuhua Luo, Xiaolei Liu, Lina Zhang, Pengyun Xie, Wending Zhou, Yue Lu, Hanhui Zhong, Xuedi Zhang, Ziying Xiong, Xiao Huang, Guixi Mo, Daqing Ma, Jing Tang
• Journal: Theranostics (Theranostics) Vol. 14 Issue 6 Pg. 2589-2604 ( 2024) ISSN: 1838-7640 [Electronic] Australia
• PMID: 38646647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The author(s).

Chemical References

• Cytokines
• Interleukin-10
• Sialic Acid Binding Immunoglobulin-like Lectins
• Siglecf protein, mouse

Topics

• Animals
• Male
• Mice
• Cytokines (metabolism)
• Disease Models, Animal
• Escherichia coli Infections (immunology)
• Immunosuppression Therapy
• Interleukin-10 (metabolism)
• Neutrophils (immunology, metabolism)
• Sepsis (immunology)
• Sialic Acid Binding Immunoglobulin-like Lectins (metabolism)
• Spleen (immunology)
• T-Lymphocytes, Regulatory (immunology)