[Yeast extract improves the inflammatory response of HepG2 cells induced by ethyl alcohol and lipopolysaccharide].

Abstract

OBJECTIVE:

To explore the ameliorative effect of yeast extract(YE) on the inflammatory response of human hepatoma cells(HepG2) induced by ethyl alcohol(EtOH) and lipopolysaccharide(LPS), and further explore the potential molecular mechanism based on Toll-like receptor 4(TLR4)/nuclear factor kappa B(NF-κB) signaling pathway.

METHODS:

HepG2 cells were induced by 50 mmol/L EtOH and 1 μg/mL LPS combined with YE intervention. The expression level of inflammatory cytokines was detected by ELISA. The expression level of TLR4 and the nuclear translocation of NF-κB were detected by immunofluorescence staining. The expression levels of TLR4, NF-κB, phospho-NF-κB-P65(P-NF-κB-p65), nucleus-phospho-NF-κB-p65(N-P-NF-κB-p65), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β) were detected by Western blot.

RESULTS:

Compared with the control group, the cells in EtOH+LPS group produced a large number of inflammatory factors and had a significant inflammatory response. YE intervention significantly alleviated EtOH+LPS induced hepatocyte inflammatory response. Further molecular mechanism studies showed that YE significantly reduced TLR4 expression level and inhibited NF-κB nuclear translocation in hepatocytes.

CONCLUSION:

YE can effectively inhibit the inflammatory response of HepG2 cells induced by EtOH and LPS, and its molecular mechanism may be related to the down-regulation of TLR4/NF-κB pathway.

• Authors: Lei Tian, Yan Zhang, Qian Cheng, Yanyang Han, Yajing Dong, Xiang Li, Hao Han
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 53 Issue 1 Pg. 66-70 (Jan 2024) ISSN: 1000-8020 [Print] China
• PMID: 38443174 (Publication Type: English Abstract, Journal Article)

Chemical References

• NF-kappa B
• Lipopolysaccharides
• Toll-Like Receptor 4
• Ethanol
• Interleukin-1beta
• Interleukin-6
• Tumor Necrosis Factor-alpha

Topics

• Humans
• Hep G2 Cells
• NF-kappa B
• Lipopolysaccharides
• Toll-Like Receptor 4
• Ethanol (toxicity)
• Interleukin-1beta
• Interleukin-6
• Tumor Necrosis Factor-alpha