Immunoprotective effects of DNA vaccine against Eimeria tenella based on EtAMA3 and EtRON2L2.

Abstract

Eimeria tenella is the most pathogenic and harmful intestinal parasitic protozoan. Recombinant DNA vaccines open options for promising strategies for preventing avian coccidiosis, replacing chemical drugs and live oocyst vaccines. Two important antigenic proteins, EtAMA3 (also known as SporoAMA1) and EtRON2L2, act together to promote the invasion of E. tenella sporozoites. In this study, a recombinant DNA vaccine, designated pcDNA3.1(+)-AR, was constructed based on EtAMA3DII, EtRON2L2D3, and EtRON2L2D4. Chickens were intramuscularly immunized with different doses (25, 50, or 100 μg) of pcDNA3.1(+)-AR to evaluate its immunoprotective effects in vivo. The chickens in the 50 μg and 100 μg groups had higher cytokine concentrations (interleukin 2, interferon-gamma, and interleukin 10), and lesion scores (81.9% and 67.57%, respectively) and relative oocyst production (47% and 19%, respectively) reduced compared with the unchallenged group, indicating partial protection against E. tenella. These results suggest that pcDNA3.1(+)-AR is a promising vaccine candidate against avian coccidiosis.

• Authors: Xiangjie Chen, Xixi Qiu, Junli Ni, Shenquan Liao, Nanshan Qi, Juan Li, Minna Lv, Xuhui Lin, Haiming Cai, Junjing Hu, Yongle Song, Lijun Yin, Yibin Zhu, Jianfei Zhang, Haoji Zhang, Mingfei Sun
• Journal: Veterinary parasitology (Vet Parasitol) Vol. 327 Pg. 110141 (Apr 2024) ISSN: 1873-2550 [Electronic] Netherlands
• PMID: 38367528 (Publication Type: Journal Article)
• Copyright: Copyright © 2024 Elsevier B.V. All rights reserved.

Chemical References

• Vaccines, DNA
• Recombinant Proteins
• Protozoan Vaccines

Topics

• Animals
• Eimeria tenella
• Vaccines, DNA
• Chickens (parasitology)
• Coccidiosis (prevention & control, veterinary)
• Recombinant Proteins
• Oocysts
• Poultry Diseases (parasitology)
• Protozoan Vaccines