Because of the mutual relationship between neural
inflammation and seizure, this study aimed to determine the effects of intracerebroventricular (ICV) injection of the steroidal and non-steroidal anti-inflammatory drugs on
pentylenetetrazol (PTZ)-induced
seizures during the estrous cycle in rats. A total of 105 adult female Wistar rats were selected and divided into seven groups, including the control (saline),
ketorolac tris
salt (7.5, 15, and 30 µg), and
methylprednisolone acetate (0.15, 0.3, and 0.6 µg), each with four subgroups (proestrus, estrus, metestrus, and diestrus) and three replicates (n=5). After a week of acclimatization, the estrous phase determination and synchronization were performed. Acute
epilepsy was inspired by the
intraperitoneal injection of 80 mg/kg of PTZ 30 min after the ICV injection of
ketorolac and
methylprednisolone acetate. The initiation time of
myoclonic seizures (ITMS), the initiation time of
tonic-clonic seizures (ITTS), seizure duration (SD), and mortality rate (MR) were measured for 30 min. Data were shown as mean±SD and analyzed using One-way ANOVA followed by Tukey-Kramer multiple comparison post hoc test (P<0.05). According to the results,
ketorolac (15 and 30 µg) and
methylprednisolone acetate (0.3 and 0.6 µg) significantly increased the ITTS and ITMS but decreased SD during the estrous cycle, compared to the control (P<0.05). Moreover, MR and SD were significantly decreased by
ketorolac (7.5, 15, and 30 µg) and
methylprednisolone (0.3 and 0.6 µg), compared to the control during the estrous cycle (P<0.05). Therefore, it seems that both
ketorolac and
methylprednisolone possess dose-dependent
anticonvulsant effects that may decrease neural
inflammation.