Abstract | BACKGROUND: METHODS: We collected nasal fluid, nasal swabs, and hair samples from 34 infants with CF during monthly clinic visits, sick visits, and hospitalizations. Nasal fluid was isolated and analyzed for cytokines. Respiratory viral detection on nasal swabs was performed using the Luminex NxTAG® Respiratory Pathogen Panel. Hair samples were analyzed for nicotine concentration by reverse-phase high-performance liquid chromatography. We compared nasal cytokine concentrations between the presence and absence of detected respiratory viruses, PEx, and smoke exposure. RESULTS: A total of 246 samples were analyzed. Compared to measurements in the absence of respiratory viruses, mean concentrations of IL-6, IL-8, TNF-α, and NE were significantly increased while IL-17A was significantly decreased in infants positive for respiratory viruses. IL-17A was significantly decreased and NE increased in those with a PEx. IL-8 and NE were significantly increased in infants with enteric pathogen positivity on airway cultures, but not P. aeruginosa or S. aureus. Compared to those with no smoke exposure, there were significantly higher levels of IL-6, IL-10, and NE in infants with detectable levels of nicotine. CONCLUSIONS: Noninvasive collection of nasal fluid may identify inflammation in infants with CF during changing clinical or environmental exposures.
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Authors | Benjamin T Kopp, Sydney E Ross, Dinesh Bojja, Lokesh Guglani, Joshua D Chandler, Rabindra Tirouvanziam, Misty Thompson, James E Slaven, James F Chmiel, Christopher Siracusa, Don B Sanders |
Journal | Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
(J Cyst Fibros)
Vol. 23
Issue 2
Pg. 219-225
(Mar 2024)
ISSN: 1873-5010 [Electronic] Netherlands |
PMID | 37977937
(Publication Type: Journal Article)
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Copyright | Copyright © 2023 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. |
Topics |
- Humans
- Cystic Fibrosis
(microbiology)
- Infant
- Male
- Female
- Cytokines
(analysis, metabolism)
- Inflammation
(diagnosis)
- Biomarkers
(analysis, metabolism)
- Leukocyte Elastase
(analysis, metabolism)
- Respiratory Tract Infections
(diagnosis, virology, microbiology)
- Nicotine
(analysis)
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