Abstract | BACKGROUND: Pro-inflammatory cytokines secreted from activated macrophages and astrocytes are crucial mediators of inflammation for host defense. Among them, the secretion of IL-1β, a major pro-inflammatory cytokine, is especially mediated by the activation of NLRP3 inflammasome. Pro-IL-1β, which is produced in response to the invaded pathogens, such as LPS, is cleaved and matured in the NLRP3 inflammasome by the recognition of ATP. Excessively activated IL-1β induces other immune cells, resulting in the up-regulation of inflammation. Therefore, regulation of NLRP3 inflammasome can be a good strategy for alleviating inflammation. OBJECTIVE: METHODS: Primary bone marrow-derived macrophages (BMDMs) and astrocytes were stimulated by LPS and ATP with the treatment of 5-methylthiopentyl isothiocyanate, a sulforaphane analogue. The secretion of pro-inflammatory cytokines was measured by ELISA, and the expression level of NLRP3 inflammasome-associated proteins was detected by western blot. The association of NLRP3 inflammasome was assessed by co-immunoprecipitation, and the formation of ASC specks was evaluated by fluorescent microscope. RESULTS: DISCUSSION AND CONCLUSION:
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Authors | Su-Bin Choi, Ji-Hye Kim, Sehee Kwon, Na-Hyun Ahn, Joo-Hee Lee, Woong-Suk Yang, Cheorl-Ho Kim, Seung-Hoon Yang |
Journal | Current pharmaceutical biotechnology
(Curr Pharm Biotechnol)
(Aug 24 2023)
ISSN: 1873-4316 [Electronic] Netherlands |
PMID | 37622701
(Publication Type: Journal Article)
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