Autophagy in combination therapy of temozolomide and IFN-γ in C6-induced glioblastoma: role of non-coding RNAs.

Abstract

Aim: We predicted the modulation of autophagy and apoptosis in response to temozolomide (TMZ) and IFN-γ based on changes in the expression of non-coding RNAs in C6-induced glioblastoma (GBM). Materials & methods: Each rat received an intraperitoneal injection of TMZ (7.5 mg/kg) and/or IFN-γ (50,000 IU). Results: The reduced expression of H19 and colorectal neoplasia differentially expressed (CRNDE) was associated with a reduction in autophagy in response to TMZ, IFN-γ and TMZ + IFN-γ therapy, whereas the decreased level of miR-29a (proapoptotic miRNA) was associated with an increase in apoptosis. Conclusion: It appears that H19 promotes switching from autophagy to apoptosis in response to combination therapy of TMZ and IFN-γ through the miR-29a/autophagy-related protein 9A (ATG9A) pathway in C6-induced GBM.

• Authors: Hamideh Bashiri, Maryam Moazam-Jazi, Mohammad Reza Karimzadeh, Saeideh Jafarinejad-Farsangi, Amirhossein Moslemizadeh, Marziyeh Lotfian, Zahra Miri Karam, Reza Kheirandish, Mohammad Mojtaba Farazi
• Journal: Immunotherapy (Immunotherapy) Vol. 15 Issue 14 Pg. 1157-1169 (10 2023) ISSN: 1750-7448 [Electronic] England
• PMID: 37584216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Temozolomide
• MicroRNAs
• Antineoplastic Agents, Alkylating

Topics

• Rats
• Animals
• Temozolomide (therapeutic use, pharmacology)
• Glioblastoma (drug therapy)
• Brain Neoplasms (drug therapy, genetics)
• MicroRNAs (genetics)
• Autophagy
• Apoptosis
• Cell Line, Tumor
• Drug Resistance, Neoplasm (genetics)
• Antineoplastic Agents, Alkylating (pharmacology, therapeutic use)