Treatment with imatinib was useful to delay the neointimal hyperplasia of aortocaval fistula in adenine-induced renal failure rats.

Abstract

PURPOSE:

The study was conducted to investigate the effect of the treatment with imatinib, a c-kit specific inhibitor, on the neointimal hyperplasia (NIH) of aortocaval fistula (ACF) in adenine-induced renal failure rats.

MATERIALS AND METHODS:

All rats were randomly assigned to 4 groups: rats were fed on a normal diet (normal group); rats were fed on a 0.75% adenine-rich diet (renal failure group). The remaining rats underwent ACF after receiving a 0.75% adenine-rich diet and received daily saline gavage (model group) or imatinib gavage (imatinib group) for 7 days after surgery. Immunohistochemical method was used to detect c-kit expression, and Elastomeric Verhoeff-Van Gieson (EVG) staining was used to observe morphological changes of the ACF. The Pearson correlation analysis was used to evaluate the correlations of c-kit expression with intimal thickness and the percentage of stenosis, respectively.

RESULTS:

The renal failure group showed positive c-kit expression on the intima of the inferior vena cava (IVC), whereas the normal group did not. Compared to the model group, intimal thickness (P = 0.001), the percentage of stenosis (P = 0.006) and c-kit expression (P = 0.04) were decreased in the imatinib group at 8 weeks postoperatively. C-kit expression was positively correlated with both intimal thickness and percentage of stenosis (intimal thickness: R = 0.650, P = 0.003; the percentage of stenosis: R = 0.581, P = 0.011) in both the model and imatinib groups.

CONCLUSION:

Treatment with imatinib, a c-kit specific inhibitor, was useful to delay the NIH of ACF in adenine-induced renal failure rats.

• Authors: Jing Du, Jiguang Song, Lina Ding, Xiaoli Fan, Lin Lin, Anzhuang Li, Liming Liang, Xianglei Kong
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 672 Pg. 10-16 (09 10 2023) ISSN: 1090-2104 [Electronic] United States
• PMID: 37331166 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Elsevier Inc. All rights reserved.

Chemical References

• Imatinib Mesylate
• Proto-Oncogene Proteins c-kit

Topics

• Rats
• Animals
• Imatinib Mesylate
• Hyperplasia
• Constriction, Pathologic
• Neointima
• Fistula
• Proto-Oncogene Proteins c-kit
• Renal Insufficiency