Gut barrier protein levels in serial blood samples from critically ill trauma patients during and after intensive care unit stay.

Abstract

PURPOSE:

In an effort to better manage critically ill patients hospitalised in the intensive care unit (ICU) after experiencing multiple traumas, the present study aimed to assess whether plasma levels of intestinal epithelial cell barrier proteins, including occludin, claudin-1, junctional adhesion molecule (JAM-1), tricellulin and zonulin, could be used as novel biomarkers. Additional potential markers such as intestinal fatty acid-binding protein (I-FABP), D-lactate, lipopolysaccharide (LPS) and citrulline were also evaluated. We also aimed to determine the possible relationships between the clinical, laboratory, and nutritional status of patients and the measured marker levels.

METHODS:

Plasma samples from 29 patients (first, second, fifth and tenth days in the ICU and on days 7, 30 and 60 after hospital discharge) and 23 controls were subjected to commercial enzyme-linked immunosorbent assay (ELISA) testing.

RESULTS:

On first day (admission) and on the second day, plasma I-FABP, D-lactate, citrulline, occludin, claudin-1, tricellulin and zonulin levels were high in trauma patients and positively correlated with lactate, C-reactive protein (CRP), number of days of ICU hospitalisation, Acute Physiology and Chronic Health Evaluation II (APACHE II) score and daily Sequential Organ Failure Assessment (SOFA) scores (P < 0.05-P < 0.01).

CONCLUSION:

The results of the present study showed that occludin, claudin-1, tricellulin and zonulin proteins, as well as I-FABP, D-lactate and citrulline, may be used as promising biomarkers for the evaluation of disease severity in critically ill trauma patients, despite the complexity of the analysis of various barrier markers. However, our results should be supported by future studies.

• Authors: Hamiyet Donmez-Altuntas, Serap Sahin Ergul, Pinar Altin-Celik, Kadir Bulut, Hamiyet Eci Roglu, Ramazan Uzen, Gulsah Gunes Sahin, Nurhayat Tugra Ozer, Sahin Temel, Turkmen Bahadir Arikan, Aliye Esmaoglu, Recep Civan Yuksel, Murat Sungur, Kursat Gundogan
• Journal: European journal of trauma and emergency surgery : official publication of the European Trauma Society (Eur J Trauma Emerg Surg) Vol. 49 Issue 5 Pg. 2203-2213 (Oct 2023) ISSN: 1863-9941 [Electronic] Germany
• PMID: 37296330 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.

Chemical References

• Citrulline
• Claudin-1
• MARVEL Domain Containing 2 Protein
• Occludin
• Biomarkers
• Lactates

Topics

• Humans
• Critical Illness
• Citrulline
• Claudin-1
• MARVEL Domain Containing 2 Protein
• Occludin
• Prospective Studies
• Biomarkers
• Intensive Care Units
• Lactates
• Prognosis