In this experiment, the effects of biofilm on neurovascular unit functions and
neuroinflammation of patients with ischemic
cerebral stroke were investigated. For this purpose, 20 adult male rats were purchased from Taconic (8 to 10 weeks old, weighing between 20 and 24g) and selected as the research objects. Then, they were randomly divided into an experimental group (10 rats) and a control group (10 rats). Ischemic
cerebral stroke rat models were established. Besides, pseudomonas aeruginosa (PAO1) was prepared manually and implanted into the bodies of rats in the experimental group. mNSS scores,
cerebral infarction area, and the release of inflammatory
cytokines of rats in the two groups were compared. Results showed that mNSS scores for rats in the experimental group at all periods were remarkably higher than those for rats in the control group (P<0.05), which demonstrated that the rats in the experimental group suffered much severer neurological impairment than those in the control group. In addition, the release of
tumor necrosis factor (TNF)-α,
interleukin (IL)-6, IL-1β,
inducible nitric oxide synthase (iNOS), and
IL-10 were all higher than those of the control group (P<0.05). The
cerebral infarction area of the experimental group at all periods was remarkably larger than that of the control group (P<0.05). In conclusion, the formation of biofilm led to the aggravation of neurological impairment and inflammatory reactions among patients with ischemic
cerebral stroke.