Abstract | AIMS: METHODS: Sprague-Dawley rats were injected with angiotensin II to induce atrial fibrillation and randomly assigned to receive SGLT2i ( n = 6) or vehicle ( n = 6). Macrophages (RAW264.7) were treated with ketone bodies; ACC1 knockdown/overexpression and malonyl-CoA overexpression were performed in vitro . The levels of inflammatory cytokines, ACC1, and malonyl-CoA were examined by ELISA. GAPDH malonylation was measured by co-immunoprecipitation. RESULTS: CONCLUSION: SGLT2i alleviates EAT inflammation by reducing GAPDH malonylation via downregulating the expression of ACC1 through increasing ketone bodies, thus attenuating atrial fibrosis.
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Authors | Lina Li, Cuncun Hua, Xiaoyan Liu, Yidan Wang, Lei Zhao, Yeping Zhang, Li Wang, Pixiong Su, Min-Fu Yang, Boqia Xie |
Journal | Journal of cardiovascular medicine (Hagerstown, Md.)
(J Cardiovasc Med (Hagerstown))
Vol. 24
Issue 4
Pg. 232-243
(04 01 2023)
ISSN: 1558-2035 [Electronic] United States |
PMID | 36938811
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the Italian Federation of Cardiology. |
Chemical References |
- Sodium-Glucose Transporter 2 Inhibitors
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Ketone Bodies
- Malonyl Coenzyme A
- Cytokines
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Topics |
- Rats
- Humans
- Animals
- Atrial Fibrillation
- Sodium-Glucose Transporter 2 Inhibitors
- Rats, Sprague-Dawley
- Inflammation
(drug therapy, prevention & control)
- Fibrosis
- Glyceraldehyde-3-Phosphate Dehydrogenases
(metabolism)
- Ketone Bodies
(metabolism)
- Malonyl Coenzyme A
(metabolism)
- Cytokines
- Adipose Tissue
(metabolism, pathology)
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