Abstract | Background:
Oral lichen planus (OLP) is a type of chronic inflammatory disorder, which represents a potential risk of malignant transformation. Understanding the mechanism of OLP-related malignant transformation could reduce the risk of cancer. Accumulating evidence indicates that the expression of succinate dehydrogenase enzyme B (SDHB) is associated with the carcinogenesis of oral squamous cell carcinoma (OSCC). However, the function and underlying mechanism of SDHB in OLP remains unknown. Methods: In this study, we examined the expression of SDHB in tissues from OLP patients and normal oral mucosa (NOM) through immunohistochemical (IHC) staining, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and western blot (WB). Adenosine triphosphate ( ATP) assay, reactive oxygen species (ROS) assay, mitochondrial membrane potential ( MMP) assay, and glucose uptake assay were used to explore the function of SDHB in mitochondrial injury and bioenergetic changes in OLP cell model and SDHB-overexpressing cells. Results: In current study, we found that the messenger RNA ( mRNA) and protein expression of SDHB was significantly decreased in OLP patients, accompanied by the accumulation of succinate. In the lipopolysaccharide (LPS) or CoCl2-stimulated OLP cell model, the expression of SDHB was decreased along with treatment time and concentration. Mechanistically, decreased SDHB enhanced hypoxia-inducible factor (HIF)-1α activity, induced mitochondrial injury, bioenergetic changes, and cytokine release. Overexpression of SDHB could reverse the above biological process and switch bioenergetic metabolism during OLP process. Conclusions: Our study suggests that SDHB reduction promotes OLP by impairing mitochondrial respiratory function.
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Authors | Hui Zhang, Beiyun Xu, Jin Liu, Bin Guo, Hongying Sun, Qiaozhen Yang |
Journal | Annals of translational medicine
(Ann Transl Med)
Vol. 10
Issue 24
Pg. 1367
(Dec 2022)
ISSN: 2305-5839 [Print] China |
PMID | 36660661
(Publication Type: Journal Article)
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Copyright | 2022 Annals of Translational Medicine. All rights reserved. |