Abstract |
Periodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the loss of alveolar bone. Periodontitis is closely related to various systemic diseases and is emerging as a global health problem. In this study, we investigated the anti-inflammatory effect of lactic acid bacteria (LAB) in vitro on Porphyromonas gingivalis (P. gingivalis) LPS-activated RAW264.7 and human gingival fibroblasts-1 (HGF-1) cells and the anti-osteoclastogenic effect of LAB on RANKL-induced RAW264.7 cells. All LAB strains (Lacticaseibacillus rhamnosus MG4706, MG4709, and MG4711) inhibited nitric oxide (NO)/ inducible nitric oxide synthase (iNOS) in P. gingivalis LPS-activated RAW264.7 cells and pro-inflammatory cytokines (IL-1β and IL-6) and matrix metalloproteinase (MMP-8 and MMP-9) in HGF-1 cells. In addition, LAB treatment inhibited osteoclastogenesis by reducing tartrate-resistant acid phosphatase (TRAP) activity and cathepsin K (CtsK) through the downregulation of nuclear factor of activated T cells cytoplasmic 1 (NFATc1) and c-fos gene expression in RANKL-induced RAW264.7 cells. Administration of MG4706 alleviated alveolar bone loss indices and reduced the gene expression of IL-1β, IL-6, MMP-8, MMP-9, and RANKL/OPG ratio in gingival tissue. In conclusion, L. rhamnosus MG4706 has the potential to alleviate periodontitis.
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Authors | Seonyoung Kim, Ji Yeon Lee, Jeong-Yong Park, YongGyeong Kim, Chang-Ho Kang |
Journal | Nutrients
(Nutrients)
Vol. 14
Issue 22
(Nov 17 2022)
ISSN: 2072-6643 [Electronic] Switzerland |
PMID | 36432555
(Publication Type: Journal Article)
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Chemical References |
- Matrix Metalloproteinase 9
- Lipopolysaccharides
- Interleukin-6
- Matrix Metalloproteinase 8
- Tartrate-Resistant Acid Phosphatase
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Topics |
- Rats
- Humans
- Animals
- Matrix Metalloproteinase 9
(genetics)
- Lipopolysaccharides
- Interleukin-6
(genetics)
- Matrix Metalloproteinase 8
- Periodontitis
(drug therapy, microbiology)
- Tartrate-Resistant Acid Phosphatase
- Inflammation
(metabolism)
- Lacticaseibacillus rhamnosus
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