Abstract | INTRODUCTION: OBJECTIVE: METHODS: The anti- elastase effect of Surfacen, rCmPI-II and the Surfacen/rCmPI-II combination was evaluated in an in vitro model of activated neutrophils, previously purified from the blood of healthy subjects. The cells were stimulated with LPS/fMLP and were incubated with different concentrations of Surfacen, rCmPI-II and the Surfacen/rCmPI-II combination. Elastase activity was measured. The interface property was determined on a Langmuir surface balance. The new index, called the abdominal adipose deposit index, was obtained by multiplying the subcutaneous fat thickness by visceral fat thickness, both measured by ultrasound. A cutoff point was established that facilitated discernment of an unhealthy phenotype: normal weight but metabolically obese, a cardiometabolic risk factor. RESULTS:
Surfacen at 10 mg/mL inhibited 71% of stimulated neutrophil elastase activity. rCmPI-II at 0.1 μM reduced 20% of elastase activity; at 200 μM-the maximum concentration evaluated-inhibition was 68%. Both products had a dose-dependent effect. The Surfacen/inhibitor combination (0.5 mg/mL/80 µM) did not affect the surfactant interface property or the inhibitory activity of rCmPI-II against human neutrophil elastase. CONCLUSIONS:
Surfacen and the rCmPI-II inhibitor have an anti- elastase effect on an activated neutrophil model. rCmPI-II does not affect Surfacen's interface property and, therefore, both can be evaluated for combined use in treating inflammatory lung diseases.
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Authors | Yuliannis Lugones-Ladrón de Guevara, Mercyleidi Díaz-Reyes, Aymara Cabrera-Muñoz, Arturo Chang-Monteagudo, Alonso Del Rivero-Antigua, Odalys Blanco-Hidalgo |
Journal | MEDICC review
(MEDICC Rev)
Vol. 24
Issue 3-4
Pg. 46-52
(Oct 31 2022)
ISSN: 1527-3172 [Electronic] United States |
PMID | 36417334
(Publication Type: Journal Article)
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Chemical References |
- Antiviral Agents
- Leukocyte Elastase
- Protease Inhibitors
- Pulmonary Surfactants
- surfacen
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Topics |
- Animals
- Humans
- Antiviral Agents
- Leukocyte Elastase
(pharmacology)
- Neutrophils
- Protease Inhibitors
(pharmacology)
- Pulmonary Surfactants
(pharmacology)
- Swine
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