The resistant cells that proliferate after
radiotherapy and
chemotherapy are primarily tumor stem cells with high stem marker expression, and their presence is the primary cause of
tumor dispersion. The Wnt signaling
receptor Frizzled family receptor 7 (FZD7) is linked to the maintenance of stem cell features as well as
cancer progression. Frizzled-7 (FZD7), a key receptor for Wnt/-
catenin signaling, is overexpressed in TNBC, suggesting that it could be a viable target for
cancer therapy. We employed bioinformatics to find the best-scoring
peptide, chemically synthesized FZD7
epitope antigen, and binding
toll-like receptor 7 agonists (T7). Under GMP conditions,
peptides for
vaccines were produced and purified (>95%). In vivo and vitro tests were used to assess
tumor cell inhibition. In vitro, the FZD7-T7 vaccination can boost the maturity of BMDC cells considerably. In mice, the FZD7 - T7
vaccine elicited the greatest immunological response. Significant
tumor development inhibition was seen in BALB/c mice treated with FZD7 - T7 in prevention experiments (P < 0.01). Multiple
cytokines that promote cellular immune responses, such as
interferon (IFN)-γ (P < 0.05),
interleukin (IL)-12 (P < 0.05), and
IL-2 (P < 0.01), were shown to be considerably elevated in mice inoculated with FZD7- T7. Furthermore, we evaluated safety concerns in terms of
vaccine composition to aid in the creation of successful next-generation
vaccines. In conclusion, the FZD7-T7
vaccine can activate the immune response in vivo and in vitro, and play a role in
tumor suppression. Our findings reveal a unique
tumor-suppressive role for the FZD7
peptide in TNBC.