Renal tissue plays a crucial function in maintaining homeostasis, making it vulnerable to
xenobiotic toxicity. Pueraria montana has more beneficial potential against the various diseases and has long history used as a
traditional Chinese medicine. But its effect against the
renal cancer not scrutinize. The goal of this study is to see if Pueraria montana can protect rats from developing kidney
tumors caused by
diethylnitrosamine (DEN) and ferric
nitrite (
Fe-NTA). Wistar rats was selected for the current study and DEN (use as an inducer) and
Fe-NTA (promoter) for induction the
renal cancer. For 22 weeks, the rats were given orally Pueraria montana (12.5, 25, and 50 mg/kg) treatment. At regular intervals, the
body weight and food intake were calculated. The rats were macroscopically evaluated for identification of
cancer in the renal tissue. The renal
tumor makers, renal parameters,
antioxidant enzymes, phase I and II
enzymes, inflammatory
cytokines and mediators were estimated at end of the experimental study. Pueraria montana treated rats displayed the suppression of renal
tumors, incidence of the
tumors along with suppression of
tumor percentage. Pueraria montana treated rats significantly (p < 0.001) increased
body weight and suppressed the renal weight and food intake. It also reduced the level of renal
tumor marker ornithine decarboxylase (ODC) and [3H]
thymidine incorporation along with suppression of renal parameter such as
uric acid, blood
urea nitrogen (BUN),
urea and
creatinine. Pueraria montana treatment significantly (p < 0.001) altered the level of phase
enzymes and
antioxidant. Pueraria montana treatment significantly (p < 0.001) repressed the level of
tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β),
interleukin-6 (IL-6) and improved the level of
interleukin-10 (IL-10). Pueraria montana treatment suppressed the level of
prostaglandin (
PGE2),
cyclooxygenase-2 (COX-2), nuclear kappa
B factor (NF-κB) and
transforming growth factor beta 1 (TGF-β1). Pueraria montana suppressed the inflammatory
necrosis, size the bowman capsules in the renal histopathology. Pueraria montana exhibited the chemoprotective effect via dual mechanism such as suppression of inflammatory reaction and oxidative stress.