Abstract |
METHODS: We studied 38 children diagnosed with JIA and 45 healthy children. We quantified GAAGs by assessing the concentration of unsaturated disaccharide units formed by digestion of isolated glycosaminoglycans with chondroitinase ABC, while COMP, YKL-40, and PDGF-BB were quantified using immunoenzymatic methods. RESULTS: Compared to the control group, GAAGs and COMP levels were significantly lower, while YKL-40 levels were higher in the blood of patients with aggressive JIA, qualified for ETA treatment. ETA therapy leading to clinical improvement simultaneously promoted normalization of COMP and YKL-40 levels, but not GAAGs. After 24 months of taking ETA, glycan levels were still significantly lower, relative to controls. GAAGs, COMP, and YKL-40 levels were significantly influenced by ADAMTS4, ADAMTS5, and PDGF-BB levels both before and during ETA treatment. CONCLUSIONS: The dynamics of changes in marker concentrations during treatment seem to indicate that measurement of COMP and YKL-40 levels can be used to assess the chondroprotective biological efficacy of therapy. In contrast, changes in GAAGs concentrations reflect systemic extracellular matrix transformations in the course of JIA.
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Authors | Klaudia Dąbkowska, Magdalena Wojdas, Kornelia Kuźnik-Trocha, Grzegorz Wisowski, Anna Gruenpeter, Katarzyna Komosińska-Vassev, Krystyna Olczyk, Katarzyna Winsz-Szczotka |
Journal | Journal of clinical medicine
(J Clin Med)
Vol. 11
Issue 17
(Aug 29 2022)
ISSN: 2077-0383 [Print] Switzerland |
PMID | 36079004
(Publication Type: Journal Article)
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