Correlation between IFNAR1 expression in peripheral blood T lymphocytes and inflammatory cytokines, tumor-infiltrating lymphocytes, and chemosensitivity in patients with colorectal cancer.

Abstract

IFN-α receptor (IFNAR) is critical for maintaining the crosstalk between cancer cells and lymphocytes. We investigated IFNAR1 expression in peripheral blood CD4+ and CD8+ T cells and explored their relationships with plasma cytokines, chemosensitivity and infiltrated T cells in the tumor microenvironment (TME) of colorectal cancer (CRC). The levels of IFNAR1, IFN-γ, and PD1 in peripheral T cells were tested using flow cytometry. Immunohistochemical staining of IFNAR1 in CRC tissues was performed. A cytometric bead array was used to determine the plasma concentrations of cytokines. In CRC patients, IFNAR1 levels were significantly increased in peripheral blood T cells, and plasma IL-6 levels were also significantly increased. Pearson correlation analysis revealed that IFNAR1 expression in CD8+ T cells was negatively associated with plasma IL-2, IFN-γ, and TNFα. IFNAR1 expression in CD4+ T cells was positively associated with TME infiltrated levels of CD8+ T cells. The levels of CD8+ T cells with IFNAR1 and plasma IFN-γ were associated with chemosensitivity. Collectively, IFNAR1 levels in CD4+ and CD8+ T cells were significantly upregulated in CRC patients and positively associated with T-cell infiltration. IFNAR1 may be a chemotherapy biomarker for predicting response.

• Authors: Lei Yang, Xiaojing Zhang, Xiaoxi Huang, Xichen Dong, Shui Jing, Yudong Zhang, Baocheng Zhao, Zhenjun Wang, Hao Qu
• Journal: Cytokine (Cytokine) Vol. 159 Pg. 156008 (11 2022) ISSN: 1096-0023 [Electronic] England
• PMID: 36063748 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.

Chemical References

• Cytokines
• IFNAR1 protein, human
• Interleukin-2
• Interleukin-6
• Tumor Necrosis Factor-alpha
• Receptor, Interferon alpha-beta

Topics

• CD4-Positive T-Lymphocytes (metabolism)
• CD8-Positive T-Lymphocytes (metabolism)
• Colorectal Neoplasms (metabolism)
• Cytokines (metabolism)
• Humans
• Interleukin-2 (metabolism)
• Interleukin-6 (metabolism)
• Lymphocytes, Tumor-Infiltrating
• Receptor, Interferon alpha-beta (metabolism)
• Tumor Microenvironment
• Tumor Necrosis Factor-alpha (metabolism)